HRP—AChE双重组化法研究大鼠脊髓小脑束乙酰胆碱酯酶阳性神经元

对脊髓小脑束各群神经元中的AChE阳性及阴性细胞加以研究。对照组2只动物分别单纯显示脊髓内AChE阳性神经元及被HRP标记的脊髓小脑束神经元。结果表明,各群脊髓小脑束起始细胞中,均含有不同程度活性的AChE。约有52％的中央颈核细胞具有较弱活性的AChE;约有55％的背核被标记神经元含有中弱活性的AChE;“脊髓边缘细胞”内有近67％的神经元具有中等以上活性的AChE。各群脊髓小脑束神经元中AChE细胞所占比例及AChE含量各有所不同,这可能与脊髓小脑各束的不同功能有关。

The Acetylcholinesterse Containing Neuros of the Spinocerebellar Tracts in the Cord of Rat by Using HRP-AChE Double Labeling Technique

12 rats were used in present study for localization of the AChE-contianing neurons of the spinocerebellar tracts in the cord. 10 cases of them, the HRP-AChE double-labeled and HRP sing-labeled neurons in these gments of the spinal cord were traced with Mesulam's histochemical techrique. AChE-containing neurons and spinocerebellar neurons in the same segment of the spinal cord are demonstrated respectively in another 2 cases. The result of present study suggests that approximate 50 per cent of the spinocerbellar neurons in rat spinal cord contain AChE, although the amounts of AChE in those neurons may be different. The population of the AChE neurons in different cell-groups of the spinocerebellar tracts would be ralated with the functional differences between cell-groups.