Determination of mutagenicity of the precipitate formed by sodium hypochlorite and chlorhexidine using the Ames test |
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| Authors: | Pranali Patil BDS MSD Anita Aminoshariae DDS MS Jarrod Harding PhD Thomas A Montagnese DDS MS Andre Mickel DDS MSD |
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| Affiliation: | 1. Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, USA;2. Department of Biostatistics, Case Western Reserve University, Cleveland, Ohio, USA |
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| Abstract: | The aim of this study was to determine the direct mutagenic potential of any precipitate formed by combining sodium hypochlorite (NaOCl) and chlorhexidine (CHX). The precipitates formed by NaOCl and CHX were dissolved in 100% dimethyl sulfoxide and cultured with mutant Salmonella Typhimurium strains. The cells were observed for reverse mutation. The numbers of positive/mutated wells were statistically compared with those in the background plates using the two‐sample proportion independent t‐test. The precipitates were not found to be significantly more mutagenic than the background plates. Within the limitations of this study, the results suggest that the precipitates formed when sodium hypochlorite and chlorhexidine contact did not show mutagenic (and are therefore carcinogenic) potential. |
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| Keywords: | Carcinogenicity chlorhexidine endodontic irrigants mutagenicity root canal |
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