| GC法测定砂仁煎剂中樟腑含量及变化 |
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| 引用本文： | 刘清华,葛尔宁.GC法测定砂仁煎剂中樟腑含量及变化[J].甘肃中医,2010,23(4):25-26. |
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| 作者姓名： | 刘清华 葛尔宁 |
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| 作者单位： | 浙江中医药大学,浙江,杭州,310053 |
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| 摘 要： | 目的：测定砂仁中樟脑在沸水后下煎药法中煎出量及变化规律。方法：采用毛细管气相色谱法，色谱柱为DB-225（0．25mm×30mm，0．25μm），柱温：程序升温，初始温度90℃，保持4分钟，以5℃／min的速率升温至140℃，再以20℃／min的速率升温至220℃，保持1分钟。氢火焰离子化检测器（FID），载气：N2，气压：0．5kPa，进样口温度：200℃，检测器温度：250℃。结果：樟脑的回归方程为：y=6509．9x＋65．5，r=0．9992；线性范围：0．21=1．25μg。结论：砂仁在沸水后下煎药法中，其樟脑在汤药中的含量在煎煮30秒时达高峰。
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| 关 键 词： | 砂仁 樟脑 毛细管气相色谱法 |
GC Method to Measure the Content and the Regularity of Camphor in Fructus Amomi Decoction |
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| LIU Qing-hua,GE Er-ning.GC Method to Measure the Content and the Regularity of Camphor in Fructus Amomi Decoction[J].Gansu Journal of Traditional Chinese Medicine,2010,23(4):25-26. |
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| Authors: | LIU Qing-hua GE Er-ning |
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| Institution: | (Zhejiang University of TCM, Hangzhou Zhejiang China 310053) |
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| Abstract: | Objective：To measure the content and its regularity of camphor in fructus amomi decoction. Methods： Capillary column gas chromatography method was used. The column was DB-225 （0.25 mm×30 m, 0.25μm）. The col- urrm oven temperature was maintained at 90℃ for 4min after injection, programmed to 140℃ with 5℃/min, and then programmed to 220℃ with 20℃/min and held in 220℃ for lmin. The detector was FID. The carrier gas was nitrogen. The pressure was 0.5kPa. The injector temperature was 200℃ and the detector temperature was 250℃. Results： The regression equation of camphor was y=6509.9x＋65.5, r=0. 9992. The linear range was 0.21-1.25μg.Conclusion： The content of camphor reach the peak at 30s in decoction after fruetus amomi was added in boiling water. |
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| Keywords: | fructus amomi camphor capillary column gas chromatography |
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