Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus |
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| Authors: | E. Sato T. Miyazawa Y. Nishimura Y. Ikeda K. Kawakami M. Mochizuki N. Yokoyama K. Maeda K. Fujita M. Kohmoto E. Takahashi T. Mikami |
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| Affiliation: | (1) Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan, JP;(2) Tsukuba Central Laboratories, Kyoritsu Shoji Co., Ltd., Ibaraki, Japan, JP;(3) Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Aichi, Japan, JP;(4) Department of Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan, JP;(5) National Institute of Animal Health, Ibaraki, Japan, JP;(6) The National Research Center for Protozoan Disease, Obihiro University, Hokkaido, Japan, JP |
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| Abstract: | Summary. We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats. Received January 14, 2000 Accepted August 4, 2000 |
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