基质金属蛋白酶9基因多态性与冠心病的关联性

目的：探讨基质金属蛋白酶9（MMP-9）基因C1562T、R279Q单核苷酸多态性(SNPs)与冠状动脉粥样硬化性心脏
病(CHD)的相关性。方法：258例CHD患者和183例健康体检者作为研究对象，采用酶联免疫吸附试验（ELISA）测定血清MMP-9水平，应用聚合酶链式反应-限制性片段长度多态（PCR-RFLP）方法对MMP-9基因C1562T和R279Q多态位点进行SNPs基因型检测，运用统计学软件SPSS 17.0分析等位基因和基因型频数分布。结果：CHD组患者血清MMP-9水平明显高于健康对照组（P<0.05）。CHD 组MMP-9基因C1562T多态位点CT+TT基因型和T等位基因频数（24.4%和11.8%）明显高于健康对照组（13.8%和7.3%）（P<0.05）。CHD组MMP-9基因R279Q多态位点G+GG基因型和G等位基因频数（60.2%和41.7%）与健康对照组（68.0%和41.5%）比较差异无统计学意义（P>0.05）。结论：CHD患者伴有MMP-9　的过度释放，MMP-9基因C1562T多态位点与CHD发病有关联，R279Q多态位点与
CHD发病无关联。

Association between matrix metalloproteomase-9 polymorphism and coronary heart disease

Objective To investigate the association between the single polymorphisms(SNPs) of MMP-9 gene C1562T,R279Q and coronary heart disease(CHD).Methods 258 patients diagnosed with CHD and 183 healthy contols were selected.The serum levels of MMP-9 were determinated using enzyme-linked immunosorbent assay(ELISA).PCR-RFLP analysis was applied to detect the C1562T and R279Q SNPs of MMP-9 gene.Statistical soft ware SPSS 17.0 was used to analyze the frequency distribution of alleles and genotypes.Results The serum MMP-9 level in CHD group was significantly higher than that in control group(P<0.05).The C1562T genotype frequencies of CT+TT genotypes and T allele were 24.4% and 11.8% in CHD group and 13.8% and 7.3% in control group,respectively,and there were significant differences between two groups(P<0.05).The R279Q genotype frequencies of AG+GG genotypes and G allele were 60.2% and 41.7% in CHD group and 68.0% and 41.5% in control group,respectively,and there were no significant differences between two groups(P>0.05).Conclusion The MMP-9 level in patients with CHD is increased and the polymorphisms in MMP-9 gene C1562T associates with the susceptibility to the CHD,but there is no association between R279Q polymorphism and CHD.