染苯大鼠脂质过氧化和抗氧化能力的观察

目的 探讨苯对大鼠脂质过氧化及酶促体系和非酶促体系的抗氧化反应的影响。方法 健康SD大鼠48只，分成4组，设一对照组，其余3组按126mg/kg(低剂量组），190mg/kg（中剂量组）、380mg/kg(高剂量组）量作灌胃染毒，于第1次染毒后第10天、第45天、第90天采血，取血清分别检测TAC、MDA含量及SOD活性。结果 低、中剂量组，在染毒第45天时，SOD活性下降，在染毒第90天时，SOD活性回升；高剂量组，随着染毒时间的延长，SOD活性及TAC含量均逐渐降低；低、中、高剂量组，MDA含量均随染毒时间的延长而升高，TAC与MDA含量均值间的相关系数r=0.7514,TAC含量与SOD活性均值间的相关系数r=0.8207。结论 苯能诱导大鼠产生脂质过氧化反应，使其抗氧化能力降低。

Survey on lipid peroxidation and antioxidative capacity in benzene exposed rats

Objective To explore the effects of benzene on lipid peroxidation and antioxidation in enzymic and non enzymic systems of rats.Method 48 SD rats were randomly divided into 4 groups,3 exposed groups were respectively given 126 mg/kg(low dose group),190 mg/kg(medium dose group),380 mg/kg benzene(high dose group)per day by gastric gavage.TAC,MDA and SOD were examined at the 10th day,45th day,90th day after exposure respectively.Results The activities of SOD in low and medium dose group were all decreased 45 days after exposure but increased 90 days after exposure;the level of TAC and the activity of SOD in high does group were gradually decreased with the time of benzene exposure;the levels of MDA in low,medium and high dose group were all increased with the time of benzene exposure.The correlation coefficients of TAC with SOD and MDA were 0 820 7(r SOD )and 0 751 4(r MDA )respectively.Conclusion Benzene exposure might induce lipid peroxidation that makes the antioxidant capacity reduced in rats.