HPLC法同时测定矮地茶配方颗粒中岩白菜素和槲皮苷

目的 建立矮地茶配方颗粒中岩白菜素和槲皮苷的含量测定方法。方法 以Diamonsil C18柱（4.6 mm×250 mm，5 μm）为色谱柱，以乙腈（A）- 0.1%（体积分数）H3PO4（B）为流动相，进行梯度洗脱，流速1.0 mL/min，检测波长256 nm，柱温30 ℃。结果 岩白菜素在0.082 5～1.32 μg之间呈良好的线性关系（r=0.999 6），平均回收率为99.45%，RSD为1.62%。槲皮苷在0.022 8～0.364 8 μg之间呈良好的线性关系（r=0.999 8），平均回收率为99.21%，RSD为1.87%。结论 该方法精密度、重复性良好，可为矮地茶配方颗粒质量控制提供参考。

Simultaneous determination of bergenin and quercitroside in Ardisiae Japonicae Herba Dispensing Granules by HPLC

Objective To establish an method for determining bergenin and quercitroside simultaneously in Ardisiae Japonicae Herba Dispensing Granules.Methods HPLC was performed on Diamonsil C18 column（4.6mm×250 mm,5 μm）at 30 ℃ with the mobile phase of acetonitrile（A）-0.1% phosphoric acid（B） for gradient elution at a flow rate of 1.0 mL/min.And the ultraviolet detection wavelength was 256 nm.Results The linear range of bergenin was 0.082 5-1.32 μg（r=0.999 6）,and the average recovery ratio was 99.45 %（RSD=1.62%）.The linear range of quercitroside was 0.022 8-0.364 8 μg（r=0.999 8）.The average recovery ratio was 99.21%（RSD=1.87%）.Conclustion The method is accurate with good repeatability,which may provide reference evidence for the quality control of Ardisiae Japonicae Herba Dispensing Granules.