VEGF和抑癌基因maspin在胃癌组织中的表达及意义

目的观察血管内皮生长因子（VEGF）和抑癌基因maspin在胃癌组织中的表达情况,研究它们与胃癌临床特征的关系。方法应用免疫组化法检测102例胃癌患者手术切除的癌组织和癌周正常组织中VEGF和maspin表达水平。结果VEGF在正常胃组织中未见表达,在胃癌组织中的表达率为50.0%,两者比较差异有显著性意义（P〈0.01）。maspin在正常胃组织中表达率为87.3%,在胃癌组织中的表达率为52.0%,两者比较差异有显著性意义（P〈0.01）。VEGF蛋白表达水平与胃癌浆膜浸润、TNM分期、淋巴结转移、5年生存率有关（P〈0.05）,maspin蛋白表达水平与胃癌浆膜浸润、淋巴结转移、5年生存率有关（P〈0.05）。结论同时检测胃癌组织中VEGF与maspin表达水平,对判断胃癌浸润转移潜能及评估患者预后有一定参考价值。

Expression of vascular endothelial growth factor and maspin in gastric carcinoma

Objective To study the expressions of vascular endothelial growth factor(VEGF)and maspin in gastric carcinoma and their relation to the clinicopathological features.Methods VEGF protein and maspin protein expression in 102 cases of human gastric carcinoma tissues and normal tissues around the foci were examined by immunohistochemical method(Envision).Results The overexpression rate of VEGF protein was 50.0%(51/102)in gastric carcinoma tissues and 0.0%(0/102)in normal tissues(P<0.01);as for maspin,the overexpression rate of maspin protein was 52.0%(53/102)in gastric carcinoma tissues and 87.3%(89/102)in normal tissues(P<0.01).The VEGF expressions were related to serosa invasion,TNM staging,lymph node metastasis and 5-year survival rate(P<0.05).There was a close relation of maspin expression with serosa invasion,lymph node metastasis and 5-year survival rate(P<0.05).Conclusion Detecting the protein levels of VEGF and maspin simultaneously in gastric carcinoma provide certain reference value in judging invasion and metastasis potential and evaluating the prognosis.