MMP-9/TIMP-1与肝癌侵袭转移及HBsAg表达的相关性研究

目的探讨基质金属蛋白酶-9（MMP-9）及组织金属蛋白酶抑制剂-1（TIMP-1）在人肝癌及癌旁组织中的表达和肝癌生物学行为及HBsAg表达之间的关系。方法应用免疫组织化学法（SP）研究40例肝癌及癌旁组织中MMP-9、TIMP-1和HBsAg的表达。结果MMP-9在肝癌和癌旁组织表达阳性率分别为72．5％和75．0％．癌组织明显减低（P〈0．01）。TIMP-1在肝癌组织和癌旁组织中的表达阳性率分别为57．5％和62．5％，肝癌中的表达较癌旁肝组织表达减低，但差异无统计学意义。转移组癌及癌旁组织MMP-9的表达显著高于无转移组（P〈0．05）：而TIMP-1则明显低于无转移组（P〈0．05）。本组肝癌患者组织HBsAg阳性率为82．5％，HBsAg阳性组中癌及癌旁组织MMP-9表达明显高于HBsAg阴性组（P〈0．05），TIMP-1在HBsAg阳性组的表达略高于HBsAg阴性组．但差异无统计学意义。MMP-9、TIMP-1在癌旁组织中与HBsAg的表达有相关性（P〈0．05），而在癌组织中则无相关性（P〉0．05）。结论MMP-9、TIMP-1在肝癌及癌旁组织中的表达明显增高，可能对于判断肝癌的浸润、转移有重要价值：HBV感染可能与MMP-9表达增加有关。

Study of the expression of MMP-9/TIMP-1 and the relationship between MMP-9/TIMP-1 and HBsAg expression in cancerous and pericancer tissues of primary hepatocarcinoma

Objective To study the expression of MMP-9/TIMP-1 in cancerous and pericancer tissues of hepatocellular carcinomar and the relationship between liver cancer biology behavior and HBsAg expression.Methods The patterns of MMP-9,TIMP-1 and HBsAg in 40 cases of PHC and their surrounding liver tissues were studied with paraffin sections using immunohistochemistry techniques.Results The positive rate of MMP-9 protein was 72.5% in HCC,and was markedly lower than in paratumor samples 75.0%(P<0.01).The positive rate of TIMP-1 protein in carcinomar and the tissue close to tumor was 57.5% and 62.5%,there was no difference between them(P>0.05).In the transfered group the expression of MMP-9 was much higher and the expression of TIMP-1 was markedly lower than in non-transfered in both cancerous and pericancer tissues of HCC(P<0.05).The positive rate of HBsAg was 82.5%,the expression of MMP-9 was much higher in the group which was infected by HBV in both cancerous and pericancer tissues(P<0.05),while TIMP-1 was a little higher(P>0.05).In pericancer tissues the expression of MMP-9 and TIMP-1 showed a correlation to HBsAg(P<0.05),while in cancerous tissues not(P>0.05).Conclusions MMP-9 and TIMP-1 are over-expressed in both cancerous and pericancer tissues of HCC,and they may have importmant value in determining whether there is metastasis or transference;HBV infection may increase the expression of MMP-9.