汉坦病毒76—118株与R22株基因重排的实验研究

目的；研究汉坦病毒汉滩型与汉城型之间能否发生基因重排，以及发生重排的频率和特点。方法：用汉坦病毒汉滩型（HTNV）76－118株与汉城型（SEOV）R22株混合感染Vero-E6细胞，空斑形成实验挑取子代克隆株24株，传供培养后用PCR方法鉴定。结果：发现24株子代病毒（70.83%)的基因组来自76－118株或R22株；1株（4.17%)HTNV型与SEOV型引物扩增均为阳性；1株（4.17%)两型病毒引物扩增均为阴性；4株（16.67%)发生了基因片段重排。结论：证实了汉坦病毒HTNV与SEOV之间可以发生重排。

Genetic Reassortment Between Hantaan Virus Strains and Seoul Virus Strains

Objective:To determine the frequency and characteristics of reassortment among viruses causing hemorrhagic fever with renal syndrome (HFRS). Methods:Mixed infections were initiated in tissue culture by using Hantaan virus strains (76-118) and Seoul virus strains (R22). Potential reassortant virus plaques were screened by multiplex RT-PCR, using primers specific for individual genome segments of each strain. Results:Most of the progeny virus plaques (70.83% of 24) had the parental genotype of 76-118 strain or R22 strain. 1 of 24 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 16.67% of 24 progeny plaques tested, involving the M and L segments. In addition, approximately 4.17% of the progeny virus plaques appeared to contain M segments originating from both parental virus strains, i.e., they were diploid. Conclusion:Genetic reassortment can occur between Hantaan virus strains and Seoul virus strains.