|
|||||
|
|
| 结核分枝杆菌MPT64诊断抗原基因的克隆及生物信息学分析 | |
| 引用本文： | 丁淑琴,李元,刘宏鹏,张怡清. 结核分枝杆菌MPT64诊断抗原基因的克隆及生物信息学分析[J]. 宁夏医学杂志, 2010, 32(10): 899-900 |
| 作者姓名： | 丁淑琴 李元 刘宏鹏 张怡清 |
| 作者单位： | 宁夏医科大学,宁夏,银川,750004 |
| 基金项目： | 宁夏医科大学校级项目 |
| 摘 要： | 目的获得结核分枝杆菌MPT64诊断抗原基因,进行DNA测序、序列特性生物学特性分析,为研究结核病诊断抗原侯选基因奠定基础。方法以结核菌标准菌株H37Rv为模板,通过PCR技术扩增出结核分枝杆菌MPT64抗原基因,将其重组到pGEM-T载体后进行序列测定和生物信息学分析。结果成功扩增出结核分枝杆菌MPT64抗原基因,测序表明该片段由699bp组成,与已发表基因核苷酸序列相比,同源性为98%,推导编码氨基酸序列同源性为98%。结论经DNA测序证实,扩增出的片段确为MPT64,该片段阅读框完整。 |
| 关 键 词： | 结核分枝杆菌 MPT64基因 克隆 序列分析 |
Cloning and sequence analyzing of the MPT64 gene from Mycobacterium tuberculosis |
|
| Affiliation: | DING Shu - qin, LI Yuan, LIU Hong -peng, et al. ( Ningxia Medical University, Yinchuan 750004, China) |
| Abstract: | Objective To obtain and analyze characters and homologies of sequence MPT64 gene,and lay bases for screening candidate antigen of Mycobacterium tuberculosis.Methods Total RNA was extracted from protoscoles of cysts.The MPT64 gene of Mycobacterium tuberculosis was amplified by PCR and recombined into pGEM-T vector for sequencing and analyzing.Results DNA sequence with an open reading frame of 699bp has been amplified successfully by PCR.Comparing with the DNA sequence published,the homologies were 98%,which deduced that the amino acid sequence of MPT64 of Mycobacterium tuberculosis were 98% identities.Conclusion The segment of amplification is MPT64 by analysis of DNA sequence,and its reading frame is integrity.It can be used as the potential gene of recombination antigen. |
| Keywords: | Mycobacterium tuberculosis MPT64 gene Cloning ： Sequencing |
| 本文献已被 维普 万方数据 等数据库收录！ | |