| Effects of tetrodotoxin monoclonal antibody on the blocking action of tetrodotoxin on sodium channels |
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| 引用本文： | 杜爱民,宋杰军,邢宝仁,沈志超,苗小飞.Effects of tetrodotoxin monoclonal antibody on the blocking action of tetrodotoxin on sodium channels[J].中国人民解放军军医大学学报,1996(3). |
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| 作者姓名： | 杜爱民 宋杰军 邢宝仁 沈志超 苗小飞 |
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| 作者单位： | Department of Medical Protection，Faculty of Naval Medicine，Second Military Medical University，Shanghai，200433 |
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| 摘 要： | ＥｆｆｅｃｔｓｏｆｔｅｔｒｏｄｏｔｏｘｉｎｍｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙｏｎｔｈｅｂｌｏｃｋｉｎｇａｃｔｉｏｎｏｆｔｅｔｒｏｄｏｔｏｘｉｎｏｎｓｏｄｉｕｍｃｈａｎｎｅｌｓＤｕＡｉｍｉｎ；ＳｏｎｇＪｉｅｊｕｎ；ＸｉｎｇＢａｏｒｅｎ；ＳｈｅｎＺｈｉ...
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Effects of tetrodotoxin monoclonal antibody on the blocking action of tetrodotoxin on sodium channels |
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| Du Aimin,Song Jiejun,Xing Baoren,Shen Zhichao, Miao Xiaofei.Effects of tetrodotoxin monoclonal antibody on the blocking action of tetrodotoxin on sodium channels[J].Journal of Medical Colleges of PLA(China),1996(3). |
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| Authors: | Du Aimin Song Jiejun Xing Baoren Shen Zhichao Miao Xiaofei |
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| Abstract: | The effect of tetrodotoxin(TTX) monoclonal antibody (McAb) 8A5 on the blocking action of TTX on sodium channels was studied by using the electrophysiological technique of whole cell recording.We found the specific TTX McAb have the following characterizations: TTX sensitivity to NG108-15 cell was high , with sodium ion current of NG108-15 cell completely blocked by only 10-6 mol/L level of TTX; when the cell was treated with TTX McAb 8A5 for 1 min and 5min, after the sodium current was completely abolished by TTX, the sodium ion current was restored to 79. 44%?. 20% and 73. 89%?. 74% (n=5) of the control values respectively; when the cell was treated for 1 min with 8A5 and TTX which had been mixed for 1 h before added,the sodium ion current was maintained at 89. 21%?. 41% (n=4) of the control. These results indicated that TTX-induced blockage on the sodium ion current could be powerfully antagonized by TTX McAb 8A5 with two distinct administering ways. |
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| Keywords: | tetrodotoxin monoclonal antibody sodium channels whole cell recording |
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