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| 三氧化二砷诱导骨肉瘤MG—63细胞凋亡的实验研究 | |
| 引用本文： | 肖涛 李康华 等. 三氧化二砷诱导骨肉瘤MG—63细胞凋亡的实验研究[J]. 湖南医科大学学报, 2002, 27(2): 111-113 |
| 作者姓名： | 肖涛 李康华 等 |
| 作者单位： | [1]中南大学湘雅二医院骨科,长沙410011 [2]湘雅医院骨科,长沙410008 |
| 摘 要： | 目的：探讨三氧化二砷（As2O3)诱导骨肉瘤MG－63细胞凋亡的作用。方法：用MTT法、透射电镜、琼脂糖凝胶电泳等方法观察As2O3诱导MG－63细胞凋亡的作用。结果：As2O3可有效地抑制MG－63细胞增殖,随着药物浓度增高其抑制作用增强,且细胞出现典型的凋亡变化。DNA琼脂糖凝胶电泳出现凋亡特征性梯形条形。透射电镜下可见细胞核仁消失,染色质浓缩聚集于核膜下。结论：As2O3可诱导骨肉瘤细胞凋亡,其作用机制有待进一步探讨。 |
| 关 键 词： | 三氧化二砷 诱导 骨肉瘤 MG－63细胞 实验 细胞凋亡 |
Experimental study on the apoptotic effect of arsenic trioxide on human osteosarcoma MG-63 cells] |
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| Tao Xiao,Kang-hua Li,Jian-zhen Fang. Experimental study on the apoptotic effect of arsenic trioxide on human osteosarcoma MG-63 cells][J]. Bulletin of Hunan Medical University, 2002, 27(2): 111-113 | |
| Authors: | Tao Xiao Kang-hua Li Jian-zhen Fang |
| Affiliation: | Department of Othopaedics, Xiangya Hospital, Central South University, Changsha 410008, China. |
| Abstract: | OBJECTIVE: To study the apoptotic effect of arsenic trioxide(As2 O3) on human ostrosarcoma MG-63 cells. METHODS: The MTT reduction test, transmission electron microscopy and agarose gel electrophoresis were applied to observe the apoptotic changes of human osteosarcoma line MG-63 induced by As2 O3. RESULTS: Typical apoptotic cellular changes were found after As2 O3 treatment. Transmission electron miroscopy showed that nucleolus disappeared and chromatin condensed under the nucleonic membrane. Agarose gel electrophorsis showed a "ladder" strand of DNA, a special phenomenon of cellular apoptosis. The above-mentioned changes were not found in the control group. CONCLUSION: As2 O3 can induce apoptosis of human osteosarcoma MG-63 cells. Further studies are to be carried out on its mechanism. |
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