| 聚合酶链反应用于鉴别恶性疟原虫不同分离株的研究 |
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| 引用本文： | 高琪,陆惠民,李菊林,唐学恒,顾亚萍. 聚合酶链反应用于鉴别恶性疟原虫不同分离株的研究[J]. 中国病原生物学杂志, 1996, 0(1) |
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| 作者姓名： | 高琪 陆惠民 李菊林 唐学恒 顾亚萍 |
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| 作者单位： | 江苏省寄生虫病防治研究所，苏州医学院 |
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| 基金项目： | 联合国开发计划署／世界银行／世界卫生组织热带病研究和培训特别规划处的资助 |
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| 摘 要： | 用恶性疟原虫主要裂殖子表面抗原基因序列（ＭＳＡ－１）为引物的聚合酶链反应（ＰＣＲ）技术检测恶性疟原虫感染。用此方法扩增实验室体外培养的ＦＣＣ１／ＨＮ株恶性疟原虫，显示１条３００ｂｐ的ＤＮＡ片断，而对实验室培养的巴布亚新几内亚ＦＣＱ－２７株则显示１条４４０ｂｐＤＮＡ条带。用此方法检测１５份采自云南中缅边界的恶性疟血样均能扩增出ＤＮＡ条带，但不同血样的ＤＮＡ条带的分子量不同，部分血样还存在１条以上的ＤＮＡ条带。在同时扩增的１０份正常人血样和１０份采自江苏间日疟流行区的现症间日疟血样中均没有发现ＤＮＡ条带。表明此方法具有很高的种和株的特异性。
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| 关 键 词： | 恶性疟原虫，聚合酶链反应 |
POLYMERASE CHAIN REACTION FOR DETECTION OF PLASMODIUM FALCIPARUM ISOLATES |
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| Gao Qi,Lu Hui-Min,Li Ju-Lin,Tang Xue-Heng,Gu Ya-ping. POLYMERASE CHAIN REACTION FOR DETECTION OF PLASMODIUM FALCIPARUM ISOLATES[J]. Journal of Pathogen Biology, 1996, 0(1) |
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| Authors: | Gao Qi Lu Hui-Min Li Ju-Lin Tang Xue-Heng Gu Ya-ping |
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| Affiliation: | Gao Qi;Lu Hui-Min;Li Ju-Lin;Tang Xue-Heng;Gu Ya-ping(Jiangsu Institute of parasitic Disease Wuxi 214064)(Suzhou Medical College) |
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| Abstract: | Polymerase Chain Reaction(PCR) using a pair of P.falciparum specific primers(PfMSA-1) was used to detect P. falciparum infection.The results showed that DNA fragment of 300bp was amplified in cultured P.falciparum isolate FCC1/HN and a DNA fragment of 440bp in cultured P. falciparum isolate FCQ-27/PNG.This technique has been used to detect blood samples collected form Yunnan endemic areas.All of the 15 microscopically confirmed P.falciparum blood samples were amplified DNA fragments,but the molecular weight of amplified DNA fragments were different in different P.falciparum cases(440bp,310bp,300bp,280bp, 270bp and 110bp).At the same time, no DNA fragments were amplified in 10 P.vivax samples collected from Jiangsu P.vivaxendemic area and 10 normal blood samples collected from Jiangsu non-endemic area. |
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| Keywords: | Plasmodium falciparum PCR |
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