| Difference in Expression of Bcl-2 and Bcl-xl Genes in Cisplatin- sensitive and Cisplatin-resistant Human in Ovarian Cancer Cell Lines |
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| 作者姓名： | 于利利 王泽华 |
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| 作者单位： | Department of Obstetrics and Gynecology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Department of Obstetrics and Gynecology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430022，China，Wuhan 430022，China |
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| 基金项目： | 国家自然科学基金，湖北省科技发展计划 |
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| 摘 要： | Apoptosisisthemainmechanismofcelldeathandisregulatedbyalargenumberofgenes.Amongthem ,theBcl 2familiesareconsideredtoplayakeyroleintheexecutionorpreventionofapoptosisinducedbygenotoxicstressthroughthealterationofapoptoticsignals.Epithe lialovariancancerist…
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| 关 键 词： | 顺铂 卵巢癌 化疗 耐药 Bcl-2基因 Bcl-xl基因 药物敏感性 细胞凋亡 |
| 收稿时间： | 21 October 2002 |
Difference in expression of Bcl-2 and Bcl-xl genes in cisplatin-sensitive and cisplatin-resistant human in ovarian cancer cell lines |
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| Yu?Lili,Wang?Zehua.Difference in Expression of Bcl-2 and Bcl-xl Genes in Cisplatin- sensitive and Cisplatin-resistant Human in Ovarian Cancer Cell Lines[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(2):151-153. |
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| Authors: | Yu Lili Wang Zehua |
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| Institution: | Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary To investigate the expression of Bcl-2 and Bcl-xl gene in sensitive (A2780) and drug-resistance (AD6) human ovarian cancer
cell lines and explore the molecular mechanism of multidrug resistance. A2780 and AD6 were detected by using DNA gel electrophoresis,
flow cytometry and RT-PCR. Our results showed that (1) “DNA ladder” was observed in A2780 and AD6 after cisplatin treatment;
(2) after 3.0, 6.0, 9.9 μg/ml of cisplatin treatment, a significant difference was noted in the rate of apoptosis between
in A2780 and AD6 (P<0.05); (3) Bcl-2 and Bcl-xl genes were overexpressed in AD6. After cisplatin treatment, the expression of Bcl-2 and Bcl-xl
genes was down-regulated in A2780 and AD6. It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells,
and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance
of human ovarian cancer.
YU Lili, female, born in 1975, Resident
This project was supported by the National Natural Science Foundation of China (No. 30070786) and a grant from the Scientific
and Technological Development Plan of Hubei Province (No. 20001P1505). |
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| Keywords: | ovarian cancer apoptosis multidrug-resistance Bcl-2 and Bcl-xl cisplatin |
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