当归拈痛汤对佐剂性关节炎大鼠肿瘤坏死因子α的影响

目的通过观察当归拈痛汤对佐剂性关节炎大鼠肿瘤坏死因子(TNF-α)的影响,评价当归拈痛汤治疗类风湿关节炎(RA)的疗效。方法将60只雄性Wistar大鼠随机分为正常组、模型组、高剂量组、中剂量组和低剂量组,采用弗氏完全佐剂造模,观察不同时间点各组大鼠的疼痛指数、肿胀指数等;在灌服当归拈痛汤18 d后,用ELIASA法检测血清TNF-α的水平。结果致炎后不同时间点各组关节肿胀度、疼痛指数比较均有显著性差异;与模型组比较,各用药组的TNF-α水平显著降低(P0.05和0.01)。结论当归拈痛汤可抑制RA大鼠过高的炎性细胞因子TNF-α分泌,能明显抑制大鼠佐剂性关节炎病变。

Effects of Danggui Niantong decoction on TNF-α in adjuvant arthritis rats

Objective It is to observe the effect of Danggui Niandong decoction on TNF-α in rats with adjuvant arthritis to evaluate this decoction's curative effect on rheumatoid arthritis.Methods Sixty male Wistar rats were randomly divided into normal group,model group,high-dose group,middle dose group and low dose group.The models were made by Freund's complete adjuvant.Pain index,swelling index and so on were observed in each group at different time points.The serum level of TNF-α was determined ELIASA after 18 days' treatment with Danggui Niantong decoction by intragastric administration.Results There were significant differences in swelling degree and pain index of joints in every group in different proinflammatory times.Compared with the model group,the level of TNF-α in every treatment group significantly lowed down(P<0.05 and P<0.01).Conclusion Danggui Niantong decoction can inhibit the secretion of inflammation factor TNFα,it can obviously inhibit the pathologic changes of adjuvant arthritis in the rats.