Establishment of cell free conversion system with biotin-labelled recombinant PrPsen expressed in E. coli |
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| Authors: | Zhang Jin Gao Jian-Mei Li Feng Han Jun Chen Lan Zhang Bao-Yun Wang Xiao-Fan Zhou Wei Liu Yong Dong Xiao-Ping |
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| Affiliation: | School of Medicine, Xi'an Jiaotong University, China. |
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| Abstract: | ![]()
Objective To report a protocol using biotin-labelled PrP protein in cell free conversion assay instead of isotope. Methods A hamster PrP protein (HaPrP) was expressed in E. coli and purified with HIS-tag affinity chromatograph. After being labelled with biotin, HaPrP was mixed with PrPSc preparation from scrapie strain 263K. Results Protease-resistant bands were detected after four-day incubation. Conclusion The new conversion model provides a reliable, easily handling, and environment-friendly method for studies of prion and transmissible spongiform encephalopathies. |
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| Keywords: | Transmissible spongiform eneephalopathies Prion Biotin Cell free conversion E. coli |
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