耳叶牛皮消水溶性部位的化学成分研究

目的对耳叶牛皮消Cynanchum auriculatum水溶性部位的化学成分进行研究。方法综合运用D-101大孔树脂柱色谱、反复硅胶柱色谱、Sephadex LH-20凝胶柱色谱等色谱技术进行分离纯化,并利用MS、NMR等现代波谱学技术对分离得到的化合物进行结构鉴定。结果从耳叶牛皮消水溶性部位分离得到15个化合物,分别鉴定为异莨菪亭(1)、异秦皮啶(2)、去乙酰基萝藦苷元(3)、吐叶醇(4)、4,4-二甲基庚二酸(5)、异落叶松脂素(6)、3-羟基吡啶(7)、3-羟基-2-甲基吡啶(8)、5-羟基-2-羟甲基吡啶(9)、2-甲基-6-(2′,3′,4′-三羟基丁基)吡嗪(10)、kiwiionol(11)、picein(12)、腺苷(13)、cynanoneside B(14)、cynanoneside A(15)。结论化合物1~2、4~13均首次从鹅绒藤属植物中分离得到,化合物15首次从该植物中分离得到。

Chemical constituents in water fraction of Cynanchum auriculatum

Objective To investigate the chemical constituents of Cynanchum auriculatum. Methods Compounds were isolated and purified by using a combination of various chromatographic techniques including D-101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and other methods. Their structures were elucidated by MS, NMR, and other modern spectroscopies. Results Fifteen compounds were isolated and identified as isocopoletin (1), isofraxidin (2), deacetylmetaplexigenin (3), vomifoliol (4), 4,4-dimethyl heptanedioic acid (5), (+)-isolariciresinol (6), 3-hydroxypyridine (7), 3-hydroxy-2-methylpyridine (8), 5-hydroxyl-2-hydroxymethylpyridine (9), 2-methyl-6-(2'',3'',4''-trihydroxybutyl)-pyrazine (10), kiwiionol (11), picein (12), adenosine (13), cynanoneside B (14), and cynanoneside A (15). Conclusion Compounds 1—2, 4—13 are isolated from the plants of genus Cynanchum Linn for the first time, while compound 15 is firstly obtained from C. auriculatum.