钛网加强的BMSC/松质骨基质修复下颌骨缺损的实验研究

目的：观察钛网加强的骨髓基质细胞(BMSC)／松质骨基质复合物修复下颌骨缺损的能力。方法：体外培养兔BMSC经扩增,诱导分化后复合同种异体松质骨基质,植入自体下颌骨缺损区,修复骨缺损,钛网固位和加强,植入6周、12周后经X线,组织学检查,观察骨形成情况。结果：骨髓基质细胞(BMSC)/松质骨基质复合物有很强的成骨作用,实验组X线观察有骨形成,组织学染色证实有新骨形成,骨磨片显示钛网和新骨获得良好的愈合。结论：钛网加强的BMSC／松质骨基质可诱导修复兔下颌骨缺损,为组织工程方法修复骨缺损提供了新的思路。

The experimental study on the effect of repairing mandibular defects with BMSCs/ cancellous bone matrix composite strengthened by titanium mesh

Objective:To observe the ability of bone-repair by bone marrow stromal cells(BMSCs)/ cancellous bone matrix(CBM) composites strengthened by titanium mesh. Method:The rabbits' BMSCs were cultured and induced in vitro. And then were combined with CBM to form the BMSCs/CBM composites, which were transplanted into the mandibular defects to observe the status of bone formation. The titanium mesh was used to strengthen the composites and to assist the fixation. Six and twelve weeks later, X-ray observation and histological examination were employed to assess the status of bone formation.Result:The composites appear to have strong bone formation effect. In the experimental group, new bone formation can be seen both by the X-ray observation and the histological examination. The good ossteo-integrity can also be observed in the interface between the mesh and the new bone tissues.Conclusion:BMSCs/CBM composites strengthened by titanium mesh can repair mandibular defects effectively and thus show a new method for repairing bone defect by the way of tissue engineering.