吴茱萸RAPD体系构建及道地性遗传背景研究

目的建立吴茱萸的RAPD分子标记技术并对吴茱萸的道地性遗传背景进行探讨。方法应用试剂盒法提取吴茱萸基因组DNA;筛选RAPD引物;对全国3个省市5个吴茱萸产区采取的48份样品进行RAPD分子标记,应用NTSYS-PC软件进行聚类分析。结果获得了吴茱萸的RAPD分子标记方法 ;通过对RAPD扩增结果进行聚类分析,发现不同品种吴茱萸差异较大,浏阳地区的吴茱萸和其他石虎品种在相似度系数为0.68时聚集为两个类群;而同一品种的石虎遗传背景差异明显,在相似度系数为0.78时,铜仁、浏阳、建德、温州的样品各自聚集在相同地域的族群。结论吴茱萸遗传背景差异明显,RAPD技术是一种可用于吴茱萸道地性遗传背景分析的有效手段。

Optimizing RAPD reaction system and authentic genuineness related genetic background of Fructus Evodia

Objective To establish molecular marker method of RAPD and to probe into the genetic background of Fructus Evodia.Methods Genomic DNA of Fructus Evodia was extracted by the DNA extraction kit method and then chose the RAPD primer;RAPD Analysis on 48 individuals of Fructus Evodia from three provinces have been done.The NTSYS-PC software was used to do cluster analysis.Results RAPD Analysis method was optimized for Fructus Evodia.RAPD Cluster analysis revealed that variety in different kinds of Fructus Evodia was significant.Evodia rutaecarpa from Liuyang and E.rutaecarpa var.officinalis from other places gathered in two populations at the similarity coefficient of 0.68.In the similarity coefficient of 0.78,the samples from Tongren,Liuyang,Jiande,and Wenzhou gathered in clusters,the same as their region ethnic groups,respectively.Conclusion Genetic background of Fructus Evodia from varies of regions is obviously different and RAPD analysis is a good manner to probe geo-authentic crude drug Fructus Evodia.