| 人载脂蛋白E基因型测定方法的建立 |
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| 引用本文： | 刘欣,郭刚,陈涛,解用虹.人载脂蛋白E基因型测定方法的建立[J].天津医科大学学报,2000,6(1):9-11. |
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| 作者姓名： | 刘欣 郭刚 陈涛 解用虹 |
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| 作者单位： | 天津医科大学基础医学院生化教研室 |
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| 基金项目： | 天津市自然科学基金资助课题(973608611) |
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| 摘 要： | 目的：建立一种准确、简便和经济的适用于大范围测定载脂蛋白Ｅ基因型的方法。方法：自中血中采用ＴＫＭ法提取人基因组ＤＮＡ，ＰＣＲ－ＲＦＬＰ确定载脂蛋白Ｅ的基因型，硝酸银染色聚丙烯酰胺凝胶，若银染效果不佳可用硝酸脱色后重染。结果：ＴＫＭ法获得ＤＮＡ在产量和质量上均能满足ＰＣＲ需求；电泳凝胶经银染可得清晰的ＤＮＡ特异带，据此能准确确定样品载脂蛋白Ｅ的基因基因型；用硝酸溶液脱色后的凝胶经重染仍可得到清晰的图
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| 关 键 词： | 载脂蛋白E 基因型 限制性片段长度多态性 |
| 文章编号： | 1006-8147(2000)01-0009-03 |
The establishment of method genotyping human apolipoprotein e |
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| LIU Xin,GUO Gang,CHEN Tao,XIE Yonghong.The establishment of method genotyping human apolipoprotein e[J].Journal of Tianjin Medical University,2000,6(1):9-11. |
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| Authors: | LIU Xin GUO Gang CHEN Tao XIE Yonghong |
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| Abstract: | Objective:A method with accuracy,economy,simple and convenience was established for the genotyping of human apolipoprotein EMethods: The genomic DNA was extracted with TKM The apolipoprotein E was genotypied by PCRRFLP in which the specific DNA was amplified and the digested DNA fragment was analized by PAGE with silver stainingResults: The extracted DNA by TKM satisfied the needs for PCR both in quantity and quality The silverstaining of the specific DNA fragment in PAGE was quite clear and special enough to genotype the apolipoprotein E Conclution: The modified PCRRFLP method for genotyping apolipoprotein E was suitable for extensive applications in the research of apolipoprotein E and association with some relative diseases |
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| Keywords: | apolipoprotein E genotype PCR-RFLP |
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