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人血浆对红细胞保存的影响
引用本文:邱艳,杨海平,查袆,李立立,李素波,汪德海,张志欣,章扬培. 人血浆对红细胞保存的影响[J]. 中国实验血液学杂志, 2005, 13(4): 692-697
作者姓名:邱艳  杨海平  查袆  李立立  李素波  汪德海  张志欣  章扬培
作者单位:1. 北京红十字血液中心,北京,100088
2. 军事医学科学院野战输血研究所,北京,100850
基金项目:本课题受国家973课题(2002CB713804),2001年北京市科技新星计划(200100775),北京市优秀人才培养专项经费资助(20042D0302049)
摘    要:
为了研究人血浆对保存的红细胞结构和功能的影响,对悬浮红细胞、洗涤红细胞以及添加不同量血浆的红细胞成分血在不同保存时间内的红细胞的功能指标,包括pH值、K^+浓度、Na^+浓度、渗透脆性、血浆游离血红蛋白、乙酰胆碱酯酶活性、ATP含量、2.3-DPG、P50含量进行了比较。结果证明,血浆有利于保存血液维持其高pH值、低K^+浓度和高Na^+浓度,有利于保存红细胞维持其ATP含量、携氧能力和红细胞变形性,但对保存血液维持低渗透脆性,对保存血液的血浆游离血红蛋白、乙酰胆碱酯酶活性、ATP含量和2.3-DPG含量无影响。结论:在红细胞成分血中加入一定量的血浆可能有利于红细胞的长期保存。

关 键 词:红细胞 血浆 红细胞保养液
文章编号:1009-2137(2005)04-0692-06
收稿时间:2005-01-11
修稿时间:2005-01-11

Effect of Human Plasma on Preservation of Red Blood Cells
QIU Yan,YANG Hai-ping,ZHA Yi,LI Li-li,LI Su-Bo,WANG De-Hai,ZHANG Zi-xin,ZHANG Yang-Pei. Effect of Human Plasma on Preservation of Red Blood Cells[J]. Journal of experimental hematology, 2005, 13(4): 692-697
Authors:QIU Yan  YANG Hai-ping  ZHA Yi  LI Li-li  LI Su-Bo  WANG De-Hai  ZHANG Zi-xin  ZHANG Yang-Pei
Abstract:
In order to study whether plasma can affect the structure and function of red blood cells during their (storage) period, the differences of pH value, concentration of K~ , Na~ , osmotic fragility, plasma hemoglobin, AchE, ATP,2.3-DPG,P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value,low K~ ,high Na~ and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.
Keywords:red blood cell   plasma   RBC preservative
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