| 衍生化-HPLC法测定人血清中丙戊酸浓度 |
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| 引用本文： | 邹小琴,毛桂福,钟小斌,黄振光,黄晓青. 衍生化-HPLC法测定人血清中丙戊酸浓度[J]. 中国当代医药, 2012, 19(23): 94-95 |
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| 作者姓名： | 邹小琴 毛桂福 钟小斌 黄振光 黄晓青 |
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| 作者单位： | 1. 广西医科大学第一附属医院,广西南宁,530021
2. 广西壮族自治区柳州市妇幼保健院,广西柳州,545001 |
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| 基金项目： | 广西壮族自治区卫生厅自筹经费科研项目 |
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| 摘 要： | 目的建立HPLC法测定丙戊酸血清浓度。方法以环己基丙酸为内标,血清样品经乙醚提取后,经2,4′-二溴苯乙酮衍生化并用HPLC法进行测定。Shim-pack CLC-C18为色谱柱（150mm×4.6mm,5μm）;流动相：甲醇-乙醇-水（70：8：22）;流速：1.1mL/min;柱温：35℃;紫外检测波长：248nm;结果血清中丙戊酸在5.0～150.5μg/mL浓度范围内呈良好线性关系（r=0.9996）,回收率为98.2%～101.3%,日内与日间RSD≤5.2%。结论本法简便、准确、专属性强,适用于临床常规监测需要。
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| 关 键 词： | 丙戊酸 衍生化 高效液相色谱法 血药浓度 |
Determination of valproic acid in serum by derivatization-HPLC |
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| ZOU Xiaoqin , MAO Guifu , ZHONG Xiaobin , HUANG Zhenguang , HUANG Xiaoqing. Determination of valproic acid in serum by derivatization-HPLC[J]. http://www.botanicus.org/, 2012, 19(23): 94-95 |
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| Authors: | ZOU Xiaoqin MAO Guifu ZHONG Xiaobin HUANG Zhenguang HUANG Xiaoqing |
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| Affiliation: | 1.The First Affiliated Hospital of Guangxi Medical University of Nanning City in Guangxi Zhuang Autonomous Region,Nanning 530021,China;2.Maternal and Child Health Hospital of Liuzhou City in Guangxi Zhuang Autonomous Region,Liuzhou 545001,China |
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| Abstract: | Objective To establish an HPLC method for determination of valproic acid in human serum.Methods Cyclohexanepropionic acid as an internal standard,serum sample was extracted with diethyl ether,2,4’-Dibromoacetophenone was selected as derivative reagent.An UV detector(248 nm) and Shim-pack CLC-C18 column(150 mm×4.6 mm,5 μm) was used,and the temperature of the column was 35℃,the mobile phase was methanol-ethyl alcohol-water(70:8:22),the flow rate was 1.1 mL/min.Results The linear range of valproic acid was 5.0-150.5 μg/mL(r = 0.999 6),with recovery of 98.2%-101.3%,The relative standard deviation(RSD) of inter-day and intra-day was less than 5.2%.Conclusion The method is convenient,accurate,specific and suitable for clinica1 routine monitoring. |
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| Keywords: | Valproic acid Derivatization HPLC Serum drug concentration |
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