| 免疫细胞化学法检测羟基喜树碱对乳腺癌MCF-7细胞的TIMP-3及空泡化作用 |
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| 引用本文： | 蒲锦兰,周滢.免疫细胞化学法检测羟基喜树碱对乳腺癌MCF-7细胞的TIMP-3及空泡化作用[J].中国当代医药,2012,19(25):7-8. |
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| 作者姓名： | 蒲锦兰 周滢 |
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| 作者单位： | 1. 四川省剑阁县人民医院药剂科,四川剑阁,628300
2. 重庆医科大学中医药学院,重庆,400016 |
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| 基金项目： | 重庆市卫生局中医药科技计划项目 |
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| 摘 要： | 目的探索羟基喜树碱对乳腺癌细胞MCF-7细胞信号转导相关基因TIMP-3表达，观察羟基喜树碱处理乳腺癌细胞MCF-7细胞形态的改变，从而探讨羟基喜树碱在乳腺癌治疗中的潜在新靶点。方法以乳腺癌细胞MCF-7为研究模型，细胞暴露于羟基喜树碱浓度为10、40、80mg／L的培养液中24h，PBS组为对照组。采用免疫组化法检测羟基喜树碱（HC胛）对乳腺癌MCF-7细胞TIMP-3表达特征及细胞形态的变化。结果HCPT对TIMP-3诱导表达作用差异不明显，但HCPT处理组细胞呈现明显的空泡化。结论HCPT可诱导乳腺癌MCF-7细胞空泡化作用。
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| 关 键 词： | 羟基喜树碱 空泡化 免疫细胞化学法 金属蛋白酶组织抑制剂-3 |
The detection of the TIMP-3 expression and the cellular vaculization induced by HCPT detected with immunocytochemical method |
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| Authors: | PU Jinlan ZHOU Ying |
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| Institution: | 1.The Pharmacy Department of the People′s Hospital of Jiange County in Sichuan Province, Jiange 628300, China; 2.College of Traditional Chinese Medical, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | Objective To explore the different concentration of HCPT effects on TIMP-3 expression in breast tomor cell line MCF-7 and the shapes of MCF-7 cells were observed in this paper. Methods MCF-7 cell cultures were exposed to HCPT at concentrations of 10, 40, 80 mg/L for 24 h. PBS group as control group. Immunocytochemical method was used to measure the TIMP-3 expression in breast tomor cell line MCF-7 and the shapes of MCF-7 cells for each sample. Results The upregulated expression of TIMP-3 in the MCF-7 cell line could not be obviously induced by different concentration of HCPT, but the obvious presentation of the cellular vaculization could be induced by the cells of the disposed guoup of HCPT. Conclusion The cellular vaculization for MCF-7 cells can be induced by HCPT. |
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| Keywords: | HCPT Cellular vaculization Immunocytochemical method TIMP-3 |
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