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| 实时荧光定量PCR检测应力性骨折新兵外周血TNFRSF10C mRNA表达 | |
| 引用本文： | 王毅铮,赵化冰,兰晓霞,张明顺,高宏生,张国辉,赵国平,尹光雅,王世鑫. 实时荧光定量PCR检测应力性骨折新兵外周血TNFRSF10C mRNA表达[J]. 中国运动医学杂志, 2011, 30(2) |
| 作者姓名： | 王毅铮 赵化冰 兰晓霞 张明顺 高宏生 张国辉 赵国平 尹光雅 王世鑫 |
| 作者单位： | 1. 武警医学院天津市职业与环境危害生物标志物重点实验室,天津,300162;武警医学院部队卫生学教研室 2. 武警医学院流行病学教研室 3. 武警医学院天津市职业与环境危害生物标志物重点实验室,天津,300162 4. 武警医学院部队卫生学教研室 5. 武警医学院天津市职业与环境危害生物标志物重点实验室,天津,300162;河北医科大学公共卫生学院 6. 天津市东丽区东丽医院 |
| 基金项目： | 武警医学院博士科研启动基金(WBS200805),武警医学院院级重点项目(WJZ2007-4) |
| 摘 要： | 目的:检测TNFRSF10C基因在应力性骨折(SF)患者与正常人之间的表达差异。方法:采用1:1配比的病例对照研究,选取入伍新兵SF病例及正常对照各10例,取受试者空腹肘静脉血,采用实时荧光定量PCR检测TNFRSF10C基因表达,结合管家基因GAPDH进行相对定量分析。结果:设计并合成了一组只特异性结合人TNFRSF10C基因的实时荧光定量PCR引物,结果未发现非特异性扩增。10例SF样本中有7例TNFRSF10C基因表达上调,上调1.4倍至6.7倍,另3例SF样本TNFRSF10C基因表达下调,分别为50%、90%和90%。结论:本研究建立的TNFRSF10C基因实时荧光定量PCR检测体系特异性好、可靠。TNFRSF10C基因表达结果为分析SF发病的分子机制及SF早期诊断提供了重要线索。 |
| 关 键 词： | 应力性骨折 外周血 TNFRSF10C基因 实时荧光定量PCR |
Detecting TNFRSF10C mRNA Expression in Peripheral Blood of Recruits with Stress Fracture |
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| Wang Yizheng,Zhao Huabing,Lan Xiaoxia,Zhang Mingshun,Gao Hongsheng,Zhang Guohui,Zhao Guoping,Yin Guangya,Wang Shixin. Detecting TNFRSF10C mRNA Expression in Peripheral Blood of Recruits with Stress Fracture[J]. Chinese Journal of Sports Medicine, 2011, 30(2) | |
| Authors: | Wang Yizheng Zhao Huabing Lan Xiaoxia Zhang Mingshun Gao Hongsheng Zhang Guohui Zhao Guoping Yin Guangya Wang Shixin |
| Affiliation: | Wang Yizheng 1,2,Zhao Huabing 1,Lan Xiaoxia 3,Zhang Mingshun 1,Gao Hongsheng 1,Zhang Guohui 2,Zhao Guoping 1,4,Yin Guangya 1,Wang Shixin 5 1 Tianjin Key Laboratory of Biomarkers for Occupational and Environmental Hazard,Medical College of Chinese People' s Armed Police Forces,Tianjin,China 300162 2 Department of Military Hygiene,China 300162 3 Department of Epidemiology,... |
| Abstract: | Objective To identify the different expression of TNFRSF10C gene between recruits with stress fracture(SF)and healthy individuals(controls).Methods Ten recruits with SF,and ten one by one matched controls were involved in this study.Their peripheral blood samples were collected and the mRNA expressions of TNFRSF10C gene in the blood were evaluated by real-time quantitative PCR and quantified according to house-keeping gene GAPDH.Specific binding TNFRSF10C gene primers were used in the PCR.Results Non-specif... |
| Keywords: | stress fracture peripheral blood TNFRSF10C gene real-time PCR |
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