RNA干扰假基因Cripto-3表达对大肠癌细胞增殖和侵袭的影响

目的：探讨RNA干扰下调假基因Cripto-3（Cr-3）对人大肠癌细胞SW480增殖和侵袭的影响。方法：将大肠癌细胞随机分为5组：空白对照组,空载对照组,siRNA转染组（5 nmol/L组、10 nmol/L和20 nmol/L组）。将Cr-3小干扰RNA（small interfering RNA,siRNA）转染人大肠癌SW480细胞后,采用实时荧光定量PCR检测Cr-3表达水平;分别采用平板克隆形成实验和Transwell法检测大肠癌细胞的增殖和侵袭能力。结果：与空白对照组和空载对照组比较,siRNA转染组Cr-3表达水平均明显下降,且呈浓度依赖性（P〈0.05）,癌细胞克隆形成和穿过滤膜的细胞均明显减少,且与浓度相关（P〈0.05）。结论：假基因Cr-3在大肠癌细胞增殖侵袭中起着重要的作用,可能是大肠癌诊疗中的一个重要靶点。

RNA interference in pseudogene Cripto-3 on proliferation and invasion of human colon cancer cell

Objective： To study the effects of RNA interference-mediated silencing of pseudogene Cripto3（Cr-3） on proliferation and invasion of colon cancer cells.Methods： The cells were divided into five groups,blank control group（Con-A group）,empty control group（Con-B group）,siRNA transfection group（5 nmol / L group,10 nmol / L group and 20 nmol / L group）.After human colon cancer cell line SW480 were transfected by Cr-3 small interfering RNA（siRNA）,the levels of Cr-3 were determined by quantitative real-time PCR.The cell growth and invasion ability was examined by clone formation in soft agar,and Transwell,respectively.Results： Compared with Con-A group and Con-B group,siRNA transfection group（5 nmol / L group,10 nmol / L group and 20 nmol / L group） had lower levels of Cr-3 mRNA expression with lower invasion ability of colon cancer cells and in a dose-dependent manner（both P 0.05）.Conclusion： Cr-3 might play an important role in the development of human colon cancer,and down-regulation of Cr-3 by its siRNA could inhibit the invasion of human colon cancer cells.