| HPLC同时测定玄参中5种成分的含量 |
| |
| 引用本文： | 张雪梅,王瑞,安睿,吴喜民,王新宏,李医明. HPLC同时测定玄参中5种成分的含量[J]. 中国中药杂志, 2011, 36(6): 709-711 |
| |
| 作者姓名： | 张雪梅 王瑞 安睿 吴喜民 王新宏 李医明 |
| |
| 作者单位： | 上海中医药大学,中药学院,上海,201203 |
| |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09103-398,2009ZX09308-003) |
| |
| 摘 要： | 目的:建立同时测定玄参药材中哈巴苷、哈巴俄苷、肉桂酸、麦角甾苷、安格洛苷C含量的高效液相色谱方法.方法:采用Agilent SB-C18色谱柱(4.6 mm ×250 mm,5 μm);流动相乙腈-0.03%磷酸水溶液,梯度洗脱;流速1 mL·min-1;检测波长210,280,330 mm;柱温30℃.结果:哈巴苷、哈巴俄苷、肉桂酸、麦角甾苷、安格洛苷C的线性范围分别为50～400,1～40,1～20,0.5～4.5,1～60 mg·L-1;哈巴苷、哈巴俄苷、肉桂酸、麦角甾苷、安格洛苷C的回收率分别为101%(RSD 0.62%),102%(RSD 0.32%),98.8%(RSD 0.48%),99.9%(RSD 1.4%),99.2%(RSD 1.1%).结论:该方法简便快速,分离效果好,灵敏度高,重现性好,可为全面控制玄参质量提供参考.
|
| 关 键 词： | 玄参 含量测定 HPLC 哈巴苷 肉桂酸 哈巴俄苷 麦角甾苷 安格洛苷C |
| 收稿时间： | 2011-02-18 |
Simultaneous determination of five constituents in Scrophularia ningpoensis by HPLC |
| |
| ZHANG Xuemei,WANG Rui,AN Rui,WU Ximin,WANG Xinhong and LI Yiming. Simultaneous determination of five constituents in Scrophularia ningpoensis by HPLC[J]. China Journal of Chinese Materia Medica, 2011, 36(6): 709-711 |
| |
| Authors: | ZHANG Xuemei WANG Rui AN Rui WU Ximin WANG Xinhong LI Yiming |
| |
| Affiliation: | Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
| |
| Abstract: | |
| |
| Keywords: | Scrophularia ningpoensis assay HPLC harpagide cinnamic acid harpagoside acteoside angoroside C |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
