(1) Department of Medicine School of Medicine, University of California, San Diego La Jolla, 92093, California, USA
Abstract:
Summary A new growth medium is specially designed for reliable and reproducible primary culture of human amniotic fluid cells. In comparison with many conventional media at 15 to 20% serum, this medium at 4% serum provides actively growing primary cultures for cytogenetic studies in less time with a larger number of metaphase cells present.