电针对SAMP8小鼠海马线粒体呼吸链酶复合体活性的影响

目的观察电针对SAMP8小鼠海马线粒体呼吸链酶复合体活性的影响,从线粒体角度探讨电针治疗阿尔茨海默病(AD)的作用机制。方法选取8月龄SAMP8小鼠16只,随机分为模型组8只,电针组8只,另选取8只8月龄的SAMR1小鼠为对照组,疗程结束后,采用Morris水迷宫检测小鼠学习记忆能力,分光光度法检测海马线粒体呼吸链酶复合体的活性。结果模型组与对照组相比,模型组鼠平均逃避潜伏期明显延长,原平台象限停留时间缩短,海马线粒体呼吸链酶复合体Ⅰ、Ⅱ、Ⅲ和Ⅳ活性降低(P<0.01,P<0.05);电针组与模型组比较,电针组平均逃避潜伏期缩短,原平台象限停留时间延长,海马线粒体呼吸链酶复合体Ⅰ、Ⅱ、Ⅲ和Ⅳ活性明显升高(P<0.01,P<0.05)。结论电针治疗AD的可能机制是提高海马线粒体呼吸链酶复合体活性,改善线粒体功能。

Effect of Electroacupuncture on Respiratory Chain Complex Enzyme Activity in SAMP8 Mouse Hippocampine Mitochondria

Objective To evaluate the effect of electroacupuncture on respiratory chain complex enzyme activity in SAMP8 mouse hippocampine mitochondria and explore from mitochondria the machanisn of therapeutic action of electroacupuncture on Alzheimer disease.Methods Sixteen 8-month-old SAMP8 mice were randomly allocated to a model group of 8 mice and an electroacupuncture of 8 mice.Eight other 8-month-old SAMP8 mice constituted a control group.Mouse learning ability and memory were assessed using a Morris water maze.Respiratory chain complex enzyme activity in hippocampine mitochondria was measured by spectrophotometry.Results The mean escape latency lengthened significantly,the time spent in the original platform quadrant shortened significantly and respiratory chain enzyme complexⅠ,Ⅱ,Ⅲ and Ⅳ activities in hippocampine mitochondria decreased in the model group of mice compared with the control group（P〈0.01,P〈0.05）.The mean escape latency shortened,the time spent in the original platform quadrant lengthened and respiratory chain enzyme complexⅠ,Ⅱ,Ⅲ and Ⅳ activities in hippocampine mitochondria increased significantly in the electroacupuncture group compared with the model group（P〈0.01,P〈0.05）.Conclusion Possible mechanisms for electroacupuncture treatment of AD are increasing respiratory chain complex enzyme activity in hippocampine mitochondria and improving mitochondrial function.