| 问号赖型钩端螺旋体内鞭毛蛋白与外膜蛋白基因融合DNA疫苗载体的构建 |
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| 引用本文： | 王敏,戴保民,游自立,方之茂,王雅静. 问号赖型钩端螺旋体内鞭毛蛋白与外膜蛋白基因融合DNA疫苗载体的构建[J]. 四川大学学报(医学版), 2002, 33(2): 169-171 |
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| 作者姓名： | 王敏 戴保民 游自立 方之茂 王雅静 |
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| 作者单位： | 四川大学华西基础医学与法医学院,钩体病研究室,成都,610041;四川大学华西基础医学与法医学院,寄生虫学教研室,成都,610041 |
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| 基金项目： | 国家自然科学基金资助 (批准号 39970 6 6 7) |
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| 摘 要： | 目的 为增强问号赖型钩端螺旋体 (钩体 0 17株 ) DNA疫苗内鞭毛蛋白基因 (fla B2 )与外膜抗原基因 (omp L1 )的免疫保护作用 ,构建一种能表达 fla B2 与 om p L1 蛋白融合蛋白的 DNA疫苗载体。方法 通过聚合酶链反应分别扩增 0 17钩体 fla B2 与 omp L1 片段并进行融合 ,获得的嵌合基因 om p L1 - fla B2 中含有 10个氨其酸的中间接头序列 ,以维持其空间构象。结果 经酶切鉴定 ,证实有一 1.8kb片段插入载体 ,其 fla B2 及 om p L1 DNA测序结果与文献报道的一致。结论 表达 0 17株钩体 fla B2 与 om p L1 抗原融合蛋白的 DNA疫苗载体构建成功
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| 关 键 词： | 问号赖型钩端螺旋体 内鞭毛 外膜 基因融合 疫苗 |
| 修稿时间： | 2001-06-27 |
Construction of DNA Vaccine Including a Chimeric Gene Encoding Flagellin and Outer Membrane Protein Antigen from Leptospira Interrogons Serovar |
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| Wang Min ,Dai Baomin,You Zili,Fang Zhimao,Wang Yajing. Research Unit of Leptospirosis,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu ,China. Construction of DNA Vaccine Including a Chimeric Gene Encoding Flagellin and Outer Membrane Protein Antigen from Leptospira Interrogons Serovar[J]. Journal of Sichuan University. Medical science edition, 2002, 33(2): 169-171 |
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| Authors: | Wang Min Dai Baomin You Zili Fang Zhimao Wang Yajing. Research Unit of Leptospirosis West China School of Preclinical Forensic Medicine Sichuan University Chengdu China |
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| Affiliation: | Wang Min *,Dai Baomin,You Zili,Fang Zhimao,Wang Yajing. * Research Unit of Leptospirosis,West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China |
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| Abstract: | Objective To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB 2) and an outer membrane protein gene (ompL 1) to enhance the protective immunity of Leptospira interrogans serova Lai (Strain 017). Methods The DNA fragments encoding flaB 2 and ompL 1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL 1-flaB 2 contained a synthetic linker of ten amino acids to sustain its space conformation. Results Identified by restriction enzyme analysis,an insert fragment of 1.8kb was demonstrated. FlaB 2 and ompL 1 respectively had the same sequence as reported by DNA sequencing analysis. Conclusion A fusion expression plasmid containing flaB 2 and ompL 1 was constructed. |
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| Keywords: | Leptospira interrogans serovor Lai Flagellin gene Outer membrane protein gene Gene fusion DNA vaccine |
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