石斛合剂对2型糖尿病模型大鼠的降糖作用及其对胰岛细胞凋亡的影响

目的:研究石斛合剂对实验性2型糖尿病模型大鼠的降糖作用和胰岛细胞凋亡影响。方法:大鼠高脂高糖饲养1个月后腹腔注射低剂量STZ(30 mg/kg)诱导实验性2型糖尿病大鼠模型,继以石斛合剂(5、10、20 g/kg)灌胃治疗,测试其降糖活性作用,用组织病理学技术观察胰岛细胞的形态结构,用MTT法和Annexin V/PI法检测胰岛细胞凋亡。结果:石斛合剂具有显著的降低血糖、血脂和糖化血清蛋白作用,可显著改善STZ造模大鼠胰腺组织结构功能,使治疗组的凋亡胰岛细胞较模型组显著减少(P0.01)。结论:石斛合剂对实验性2型糖尿病模型大鼠具有的降糖作用以及保护和修复胰腺组织的结构和功能。

Effect of Dendrobium Mixture on Hypoglycemic and the Apoptosis of Islet in Rats with Type 2 Diabetic Mellitus

Objective:To study the effect of Dendrobium mixture on hypoglycemic and the apoptosis of islet in rats with type 2 diabetic mellitus.Methods:Type 2 diabetes mellitus models were induced by high sugar and fat diet and low dose intraperitoneal injection of streptozotocin(STZ) in rats,and treated with Dendrobium mixture(5,10,20 g/kg)by intragastric administration.Observed islet cell morphology with histopathological techniques and tested the apoptosis of islet cells by MTT and Annexin V/PI method.Conclusion:Dendrobium mixture could reduce the levels of blood glucose,triglyceride and glucosylated serum protein effectively and significantly improve the modeling structure and function of rat pancreatic tissue.The apoptotic islet cells was significantly reduced(P<0.01)in treatment group compared with the model group.Results:Dendrobium mixture have a hypoglycemic effect on rat models of type 2 diabetes.It can protect and restore the structure and function of pancreatic tissue.