铜绿假单胞菌随机扩增多态性DNA基因分型及耐药性分析

目的 了解铜绿假单胞菌(Pseudomonas aeruginosa,PA)的基因分型及克隆菌株对耐药率的影响.方法 对69株PA进行随机扩增多态性DNA基因分型和纸片琼脂扩散法耐药检测,结合基因分型结果进行耐药性分析.结果 69株PA基因分型36型,有47株菌是克隆菌株.药敏实验结果显示,基因分型后PA对哌拉西林、头孢吡肟、头孢他啶、亚胺培南、美罗培南、头孢哌酮/舒巴坦、阿米卡星、环丙沙星、多黏菌素B、氨曲南的耐药率分别为27.3%、36.4%、27.3%、59.1%、54.6%、45.4%、31.8%、31.8%、0.0%、40.9%.结论 在检测耐药率时应结合基因分型排除克隆菌株的影响,为当地提供合理的抗生素敏感性指南.

Random amplified polymorphic DNA technique of Pseudomonas aeruginosa and analysis drug-resistance

Objective To investigate the genotypes of Pseudomona aeruginosa and the influence of colonizing strains to drug resistance. Methods Using randomly amplified polymorphic DNA technique for gene classifying and disk diffusion test for drug-resistance test classifying. Then, 69 isolated strains were analyzed for drug-resistance linking genotypes. ResultsResults demonstrated that genotypes were distinguished 36 and colonizing strains were 47 among the 69 Pseudomonas aeruginosa strains. A relatively high resistant rates of Pseudomonas aeruginosa strains to common antibiotics were found: pipenacillin 27.3%, cefepime 36.4%, ceftazidime 27.3%, imipenem 59. 10%, meropenem 54.6%, sulbactam/cefoperazone 45.4 %, amikaein 31.8%, ciprofloxacin 31.8 %, polymyxin B 0.0%, aztreonam 40.9%. Conclusions These data suggest that we should be exclude colonizing strains linking genotypes in detecting the rate of drug resistance for developing appropriate antimicrobial susceptibility guidelines in local region.