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| 反复种植失败妇女“种植窗”时期子宫内膜组织的细胞骨架蛋白质的差异表达 | |
| 引用本文： | 刘倍余,李洁,黎明涛,胡坤华,夏婷婷,徐少元. 反复种植失败妇女“种植窗”时期子宫内膜组织的细胞骨架蛋白质的差异表达[J]. 生殖医学杂志, 2014, 0(11): 907-912 |
| 作者姓名： | 刘倍余 李洁 黎明涛 胡坤华 夏婷婷 徐少元 |
| 作者单位： | 中山大学附属第一医院妇产科生殖中心;中山医学院蛋白组研究中心;中山大学附属第三医院生殖与不孕专科;湖北十堰市人民医院生殖中心; |
| 基金项目： | 广东省科技计划项目(2009B030801022);国家自然科学基金(2008-30872762) |
| 摘 要： | 目的探讨体外受精-胚胎移植治疗周期中反复种植失败(RIF)和已生育正常妇女"种植窗"时期(LH+6~LH+9d)子宫内膜组织蛋白质表达的差异。方法选择RIF妇女和正常对照妇女各4名分为实验组和对照组。采用胶内差异双向电泳、(2D-DIGE)和基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)技术鉴定子宫内膜组织的差异表达蛋白质。结果两组之间获得16个差异表达蛋白质(其中6个下调表达,10个上调表达),其中10个参与细胞骨架构成,分别是:波形蛋白、埃兹蛋白、Septin 2蛋白、radixin蛋白、纽蛋白、角蛋白8、角蛋白10、角蛋白18、角蛋白19及肌动蛋白。结论本研究结果证实"种植窗"时期RIF妇女子宫内膜组织存在多个细胞骨架蛋白质表达的异常,可能是子宫内膜细胞"质膜转换"缺陷和胚胎种植失败的重要原因。 |
| 关 键 词： | 骨架蛋白质 种植窗 子宫内膜容受性 反复种植失败 |
Differential expression of cytoskeleton proteins in endometrium during implantation window of women with recurrent implantation failure |
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| Affiliation: | LIUBei-yu, LI Jie , LI Ming-tao , HUKun-hua , XIA Ting-ting , XUShao-yuan (1. Reproductive Medicine Center, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080 2. Center for Proteomics ,School of 3. Reproductive Medicine Center, Guangzhou 510080 Preclinical Medicine of Sun Yat-sen the Third Affiliated Hospital University ,Guangzhou 510080 of Sun Yat-sen University, 4. Reproductive Medicine Center, Hubei Shiyan People ＇ s Hospital, Shiyan 442000) |
| Abstract: | Objective： To analyze the differential expression of proteins in endometrium during ＂implantation window＂ of women with repeated implantation failures（RIF）. Methods： The endometrial samples were collected in mid luteal phase（LH＋6 to LH＋9）by micro biopsies. Four women with RIF and 4 controls were selected for the study and control group. Differential expression of proteins was analyzed by using two-dimensional difference gel electrophoresis （2D-DIGE）followed by matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）analysis. The protein identification of peptide fragments was performed in local NCBI and online MASCOT databases. Results： Compared to control women, 16 proteins were differentially expressed in endometrium during ＂implantation window＂ of women with RIF. Among them, 10 proteins were up-regulated and 6 down- regulated. Biologic functions of 9/16 proteins were involved in c3）toskeleton structure. Conclusions. The differential expression of cytoskeleton proteins in women with RIF indicates that abnormal ＂plasma membrane transformation＂ may contribute to embryo implantation failure. |
| Keywords: | Structure protein Implantation window Endometrial receptivity Repeatedimplantation failure |
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