高效液相色谱法测定复方己唑乳膏中己烯雌酚、氯霉素和甲硝唑的含量

目的 建立高效液相色谱测定复方己唑乳膏中己烯雌酚、氯霉素和甲硝唑含量的方法.方法 采用高效液相色谱法,色谱柱为Diamonsil C18 column（250 mm×4.6 mm,5μm）;流动相为甲醇-乙腈-水（55：5：40）;流速：1 mL·min-1;测定波长252 nm;柱温35℃;进样20μL.结果 该方法不受处方中基质干扰.己烯雌酚、氯霉素、甲硝唑的线性范围分别为0.016 8～0.184 8μg（r=0.999 8,n=6）、1.593 6～17.529 6 μg（r=0.999 9,n=6）、1.587 2～17.459 2μg（r=0.999 8,n=6）,平均回收率分别为99.5%、99.8%、99.9%,RSD分别为0.84%、1.2%、1.1%（n=9）.结论 该方法操作简便,灵敏度高,重现性好,可用于复方己唑乳膏中己烯雌酚、氯霉素和甲硝唑的含量测定.

Simultaneous determination of diethylstilbestrol chloramphenicol and metronidazole in compound Jizuo cream by HPLC

Objective To establish an HPLC method for the simultaneous determination of diethylstilbestrol chloramphenicol and metronidazole in compound Jizuo cream.Methods The column was Diamonsil C18（250 mm ×4.6 mm,5 μm）,the mobile phase was composed of methanol-acetonitrile-water（55∶5∶40）,and the flow rate was 1 mL·min-1.The detective wavelength was 252 nm,the column temperature was 35 ℃,and the injection volume was 20 μL.Results The linear ranges of diethylstilbestrol,chloramphenicol and metronidazole were 0.016 8-0.184 8 μg（r=0.999 8,n=6）,1.593 6-17.529 6 μg（r=0.999 9,n=6）,and 1.587 2-17.459 2 μg（r=0.999 8,n=6） respectively.The average recoveries of diethylstilbestrol,chloramphenicol and metronidazole were 99.5%,99.8%,and 99.9%,while RSD was 0.84%,1.2%,and 1.1%（n=9）.Conclusion The method is simple and accurate,highly sensitive and repeatable,which can be used to control the quality of compound Jizuo cream.