B-myb在人肝细胞癌中表达的生物学意义及与cyclin D1相关性的研究

目的：研究人肝细胞癌（hepatocellular carcinoma，HCC）组织中B—myb及细胞周期素D1（cyclin D1）的表达，探讨其与肿瘤发生发展的关系。方法：采用免疫组化法检测60例肝癌组织及癌旁肝组织中B-myb和cyclin D1的表达情况。结果：B—myb、cyclin D1在癌组织中的阳性率分别为56．67％和50％，而在癌旁组织中的阳性率分别为36．67％和31．67％，癌组织与癌旁组织比较差异有统计学意义（P〈0．05）。B-myb在人肝癌组织中的表达与临床分期、肝外转移、术后复发及肿瘤个数相关，而与门静脉癌栓、肿瘤直径、血清AFP水平及肿瘤分化程度无明显相关性。cyclin D1在人肝癌组织中的表达与临床分期、门静脉癌栓、肝外转移、术后复发、肿瘤个数及肿瘤分化程度相关，而与肿瘤直径及血清AFP水平无明显相关。在癌组织中B-myb与cyclin D1的表达呈正相关。结论：肝癌组织中B—myb及cyclin D1高表达，可促使肝癌细胞增殖，与肝癌的发生发展密切相关。

Biological significance of the expression of B-myb in hepatocellular carcinoma and the correlation with cyclin D1

Objective: To study the expression of the B-myb and cyclin D1 in human hepatocellular carcinoma(HCC) tissue and explore their correlation with initiation and progression of HCC.Methods: The expressions of B-myb and cyclin D1 in 60 cases of HCC tissue and the corresponding pericancerous liver tissue were detected by immunohistochemical method.Results:The positive rates of B-myb and cyclin D1 in the HCC tissue were higher than those in the pericancerous liver tissue(56.67% vs 36.67%,and 50% vs 31.67%,P<0.05) respectively.The expression of B-myb in the HCC tissue significantly correlated with the clinical stage,the extrahepatic metastasis,post-operative recurrence,and the number of tumor nodes but not correlated with the tumor thrombus in portal vein,the tumor diameter,the serum level of alpha-fetoprotein(AFP),and tumor differentiation.The expression of cyclin D1 in the HCC tissue was significantly associated with the clinical stage,the tumor thrombus in portal vein,extrahepatic metastasis,post-operative recurrence,the number of tumor nodes,and the differentiation of tumor but not associated with the tumor diameter and the serum level of AFP.The expression of Bmyb had positive correlation with expression of cyclin D1 in HCC tissues.Conclusion: The overexpressions of B-myb and cyclin D1 in HCC tissue may contribute to the proliferation of hepatoma cells,and have close correlation with the initiation and progression of HCC.