大鼠肾移植1000例经验总结

目的：对大鼠肾移植进行经验总结以指导实验与临床研究。方法：作者在2004-01/2009-07海德堡大学移植中心期间,采用大鼠肾动、静脉与腹主动脉、腔静脉行端侧吻合。对于急性肾移植模型,输尿管直接植入膀胱内;对于慢性肾移植模型,输尿管采用端端吻合法。术中直接切除对侧肾脏,完成大鼠肾移植1000例,并对其进行总结。结果：只要供体肾脏冲洗良好,吻合时暖缺血时间小于30min,手术时间60min左右,吻合技术娴熟,移植成功率达98%以上。结论：该实验模型安全、方便、可行。肾移植成功的关键是手术技术。

Kidney transplantation in rats：Experience of 1000 cases

OBJECTIVE：To review and analyze our experience in rat kidney transplantation which provides guidance for experimental and clinical studies. METHODS：Between January 2004 and July 2009,about 1000 rat kidneys were transplanted in the research facilities of the Department of General and Transplantation Surgery,Ruprecht-Karls-University,Germany. Briefly,the renal grafts＇ artery and vein were both anastomosed in an end-to-side technique with running sutures to the recipients＇ abdominal aorta and vena cava,respectively. In the acute and chronic kidney transplantation model the ureter was implanted either directly into the bladder or an end-to-end ureteral anastomosis was performed,respectively. Recipients were nephrectomized bilaterally. RESULTS：Flushed donor kidneys were transplanted with a warm ischemia time of less than 30 minutes and a total operative time of about 60 minutes. The procedure was successful in more than 98% of cases. CONCLUSION：The kidney transplantation model described here is safe,convenient and feasible; however,microsurgical technique requires a skillful surgeon who is the key factor for successful surgical research.