骨关节炎软骨中衰老相关β-半乳糖苷酶和c-Fos蛋白的表达

背景：文献在对软骨细胞衰老的研究中,常以衰老相关β-半乳糖苷酶的表达作为其细胞老化的一个指标,而c-Fos在骨关节炎软骨中表达情况罕见报道。目的：观察β-半乳糖苷酶、c-Fos蛋白在正常关节软骨和骨关节炎不同退变程度关节软骨中表达水平的差异。方法：选取正常关节软骨标本10个,骨关节炎软骨标本26个,根据大体观察凿取正常和骨关节炎不同退变严重程度软骨块50个,再根据关节软骨改良Mankin病理评分法分为正常关节软骨组、轻度退变组、中度退变组、重度退变组。采用免疫组织化学SABC法,计算各组标本中软骨细胞β-半乳糖苷酶、c-Fos蛋白染色阳性细胞率,比较各组中二者阳性细胞率的差异性并分析其与关节软骨不同退变程度之间的相关性。结果与结论：随着衰老软骨细胞阳性率逐渐增加,软骨退变程度逐渐加重,提示软骨细胞衰老与骨关节炎病程进展有关,软骨细胞衰老是骨关节炎可能的发病机制之一。c-Fos蛋白与骨关节炎软骨退变严重程度和软骨细胞衰老均无直接相关性,但可能在软骨退变早中期促进软骨细胞代偿性分裂增殖活跃。

Expression of senescence-associated beta-galactosidase and c-Fos protein in osteoarthritic cartilage

BACKGROUND：The expression of senescence-associated β-galactosidase （SA-β-gal） serves as a marker of cell senescence,while the expression of c-Fos in cartilage of osteoarthritis is rare.OBJECTIVE：To study expression differences of SA-β-gal and c-Fos protein in normal and impaired cartilage of osteoarthritis.METHODS：Ten normal cartilages and 26 cartilage of osteoarthritis were selected.On the basis of improved Mankin pathological score,50 cartilage specimens to divide into normal,mild impaired,medium impaired and severe impaired groups.The expression of SA-β-gal and c-Fos protein in the cartilage specimens were detected by SABC method.The difference and correlation between the percentage of the positive chondrocytes and the different impaired cartilage were analyzed.RESULTS AND CONCLUSION：The cartilage degeneration aggravated with increasing of SA-beta-gal positive chondrocytes,suggesting that chondrocyte senescence has contacted with osteoarthritis.The expression of c-Fos protein shows no direct correlation with the severity of osteoarthritis and chondrocyte senescence.The findings demonstrated that chondrocyte senescence may be one of pathogenesis of osteoarthritis,which promotes compensate for cell division in the early and medium stage of osteoarthritis.