动脉粥样硬化斑块形成时血管平滑肌细胞增生相关基因的cDNA全 …

目的 克隆动脉粥样硬化斑块形成血管平滑肌细胞（ＳＭＣ）增生相关基因的ｃＤＮＡ全长,并对其功能进行初步探讨。方法 选用氧化低密度脂蛋白作为刺激物作用于培养的人主动脉ＳＭＣ,利用减除杂交技术构建减除文库,克隆相关基因片段,在全长文库构建的基础上筛选相关基因的ｃＤＮＡ全长。并进行原核系统内蛋白表达及量效关系检测。结果 发现了４个新基因片段,克隆了一个新基因ｃＤＮＡ全长,该基因在原核系统内有约相对分子质量

Cloning whole length cDNA of related genes responsible for smooth muscle cells proliferation in atherogenesis and study on its function

OBJECTIVE: To clone whole length cDNA of the related genes responsible for vascular smooth muscle cell (SMC) proliferation in atherogenesis, and to study its function. METHODS: ox-LDL was added as a stimulant to the SMC culture medium. Subtractive library was established using subtractive hybridization technique in order to clone the related genes fragments. With the whole length cDNA library established, the whole length cDNA of the related gene was cloned. The protein expressed was studied. RESULTS: 4 new gene fragments and one whole length cDNA were cloned. The new cloned gene is able to express a protein of about 44000 daltons and closely related to the activity of ox-LDL. CONCLUSIONS: The new cloned gene is considered responsible for SMC proliferation.