孕乳期补充维生素D对仔鼠生长发育及细胞免疫功能的影响

目的 研究孕乳期补充维生素D(VitD)对仔鼠生长发育及细胞免疫功能的影响.方法 选用120只小鼠依据VitD治疗剂量不同分为高、中、低剂量组(各30只),分别给予VitD滴剂3.44、1.72、0.86 IU,并设置对照组(30只)不给予VitD.观察幼鼠体质量、外周血25-羟维生素D325-(OH) D3],分别测定细胞免疫功能(迟发性变态反应实验)、体液免疫功能[包括抗体生成细胞数和半数溶血值(HC50)测定]、单核-巨噬细胞吞噬功能(包括小鼠腹腔巨噬细胞吞噬鸡红细胞实验和碳廓清实验),并进行T淋巴细胞亚群流式细胞术测定.结果 随着VitD剂量的增加,子鼠外周血中25-(OH)D3及钙离子水平逐渐升高,与对照组比较,差异均有统计学意义(P＜0.05);体质量及体长逐渐增加,各剂量组间比较,差异无统计学意义(P＞0.05).与对照组比较,高剂量组、中剂量组足趾肿胀度、吞噬百分率、抗体生成细胞数、血清溶血素HC50均增加,吞噬指数均减低,差异均有统计学意义(P＜0.05).随VitD剂量的增加,CD4+、CD8+T淋巴细胞数量和CD4+/CD8+逐渐增加.结论 孕乳期补充VitD可促进子代小鼠的生长发育,具有增强机体免疫功能的作用.

Effects of Vitamin D on growth and immune function in neonatal mice

Objective To study the effects of Vitamin D (VitD) supplementation on growth and immune function in neonatal mice.Methods A total of 120 mice were divided into four groups (30 mice in each group) according to dose of VitD.The high-dose group,medium-dose group and low-dose group was given 3.44,1.72,0.86 IU VitD drops,respectively.The control group was not treated with VitD drops.Rat body weight,level of peripheral blood 25-(OH)D3 were observed.The cellular immune function (determined by using delayed hypersensitivity reaction experiment),humoral immune function (assessed by antibody producing cells counts and HC50 determination) and mononuclear-macrophage phagocytic function (assessed by mouse peritoneal macrophage phagocytosis of chicken red blood cells test and carbon clearance test) were detected.The flow cytometry assay was carried out to differentiate T lymphocyte subsets.Results With the increase of dose of VitD,levels of peripheral blood 25-(OH)D3 and calcium ion were gradually increased,there were statistically significant differences when compared with the control group(P＜0.05);the body weight and body length were gradually increased,while no statistically significant difference was found among the groups treated with VitD(P＞0.05).Compared to the control group,the toes swelling,phagocytic percentage,number of antibody producing cells,serum soluble HC50 in the high-dose group and medium-dose group were increased significantly,while carbon clearance test phagocytic index were decreased significantly (P＜0.05).With the increase of dose of VitD,the number of CD4+,CD8+ T lymphocytes and the CD4+/CD8+ ratio were gradually increased.Conclusion VitD could promote the growth and development of offspring mice,and enhance the immune function of the body.