老年汉族人群KL基因G-395A多态性与冠状动脉粥样硬化性心脏病及胱抑素C水平的相关性分析

目的 研究华中地区老年汉族人群Klotho(KL)基因的G-395A位点多态性与冠状动脉粥样硬化性心脏病(以下简称冠心病)及胱抑素C(Cys-C)水平的相关性.方法 选取该院老年科(年龄大于或等于60岁)汉族冠心病患者138例(冠心病组),无冠心病老年人140例(对照组)作为研究对象,用TaqMan基因探针法分别检测两组KL基因G-395A位点的基因型,并分析该位点基因多态性与冠心病及血清Cys-C水平的相关性.结果 与对照组相比,冠心病组KL基因的G-395A位点GG基因型频率明显增高(P＜0.05),AA基因型和AG基因型的频率差异无统计学意义(P＞0.05).无论在对照组还是冠心病组,GG基因型的人Cys-C水平均高于AA和AG基因型.结论 在华中地区老年汉族人群中,KL基因的G-395A位点为GG基因型的人患冠心病的风险较高.KL基因G-395A位点的基因突变可能通过影响血Cys-C水平,抑制动脉粥样硬化的发生发展.

Association of KL gene G-395A polymorphisms with coronary heart disease and serum cystatin C level in the elderly Chinese Han population in central China

Objective To investigate the single nucleotide polymorphism at G-395A site of the Klotho (KL) gene and to analyze its correlation with the coronary heart disease(CHD) and serum Cystatin C(Cys C) level in the elderly Chinese Han population in central China.Methods A case-control study was conducted in 278 elderly Chinese Han population in this department,who were divided into CHD group(138 cases)and control group(140 cases) according to bear angiography coronary or not.G-395A polymorphism of KL gene was determined by TaqMan Gene probe method,and the relationship between G-395A polymorphism and coronary heart disease and serum Cys-C level was analyzed.Results Compared with the control group,the frequency of GG genotype of G-395A in CHD group was significantly higher,and the frequency of AA genotype and AG genotype was not statistically significant.The levels of Cys-C in the GG genotype were higher than those in the AA and AG genotypes both in the control group and coronary heart disease group.Conclusion In the elderly Han population in central China,the risk of suffering coronary heart disease is higher among the GG genotype of the G-395A locus of the KL gene.KL gene G-395A site gene mutation may affect the development of atherosclerosis by affecting blood Cys-C levels.