TIGAR调节肺癌细胞的增殖和侵袭能力研究 |
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引用本文: | 赵明,冯婧,刘勇,杨玲麟.TIGAR调节肺癌细胞的增殖和侵袭能力研究[J].国际检验医学杂志,2016(6):754-755. |
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作者姓名: | 赵明 冯婧 刘勇 杨玲麟 |
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作者单位: | 泸州医学院附属医院肿瘤科,四川泸州,646000 |
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摘 要: | 目的探讨P53下游基因TIGAR在肺癌细胞A549增殖、迁移及侵袭中的作用。方法采用siRNA技术在A549细胞中干扰TIGAR的表达,细胞计数试剂盒(CCK-8)检测细胞增殖,小室法检测细胞迁移,肿瘤细胞侵袭实验检测细胞侵袭,免疫印迹杂交检测相关蛋白水平变化。结果在A549细胞中成功干扰TIGAR后,细胞增殖显著降低(P0.05),细胞迁移和侵袭能力显著减弱,侵袭相关蛋白基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)的表达量均下调。结论 TIGAR促进肺癌细胞A549的增殖,并促进细胞的迁移和侵袭能力。
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关 键 词: | TIGAR A549 增殖 迁移 侵袭 |
TIGAR promotes proliferation and invasiveness of lung cancer cells |
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Abstract: | Objective To study the role of P53 target gene TIGAR on proliferation ,migration and invasion of lung cancer cells . Methods siRNA was introduced to knock down TIGAR in A 549 cells .Proliferation was detected by CCK-8 .The ability of migra-tion and invasion was measured by and ,respectively .was used to evaluate the expression levels of related proteins .Results Knock-down of TIGAR reduced the proliferation rate (P< 0 .05) ,inhibited the ability of migration and invasion ,decreased expression levels of MMP-2 and MM P-9 .Conclusion TIGAR promotes proliferation ,migration and invasiveness of lung cancer cells . |
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Keywords: | TIGAR A549 proliferation migration invasion |
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