硝酸纤维素膜上化学药物交联抗原在斑点酶免疫法中的应用

在硝酸纤维素膜上用４０％酒精直接交联方式和自然渗滤吸附方式固定抗原后，分别作斑点酶免疫法检测血清中的ＨＣＭＶ－ＩｇＧ。结果显示：前者的重复性优于后者。酒精并联抗原斑点酶免疫法与ＥＬＩＳＡ法比较，血清中ＨＣＭＶ－ＩｇＧ检测的一致率为９３．２％，阳性血清，弱阳性血清和阴性血清的检测率无显著性差异。

Application of antigen linked with chemicals on nitrate cellulose membrane for dot-enzyme immunoassay]

HCMV-IgG in sera of pregnant women was detected by means of dot-enzyme immunoassay (DEIA), in which antigen was cross-linked on nitrate cellulose membrane through chemical reagent. The results showed better repetition by DEIA, compared with ELISA, and no significant differences were noted between the two methods in the rates of detecting HCMV-IgG in positive, weak positive and negative sera. The rate of agreement was 93.2%. In examining the sera which had been examined by ELISA for three times with the same, results the sensitivity, specificity, index and efficiency of diagnosis by DEIA were 100%, 96.6%, 196.6% and 98.7% respectively. Since this method is convenient, fast, of good repetition, economical and its diagnosis index meets the application criterion, it is more suitable for clinical laboratories.