白芍总苷对小鼠溃疡性结肠炎的作用

目的探讨白芍总苷（TGP）对小鼠溃疡性结肠炎（UC）的作用及机制。方法将48只雄性BALB／c小鼠随机分为6组（n=8）：正常对照组、模型组、柳氮磺胺吡啶（SASP）组（100mg·kg-1·d-1）以及低、中、高剂量TGP（60、120、240mg·kg-1·d-1）组。除正常对照组外，其他组小鼠均以恶唑酮灌肠造模。灌肠1d后，SASP组和TGP组开始给予相应的药物灌胃治疗3d，同时进行疾病活动指数（DAI）评分。处死小鼠后，行结肠大体损伤和组织学损伤评分，以ELISA方法检测结肠黏膜中肿瘤坏死因子-a（TNF-a）水平，以免疫组织化学方法检测结肠黏膜中核因子-kB（NF—kB）p65的表达。结果模型组小鼠DAI评分明显高于正常对照组（P〈0．01），与低剂量TGP治疗组相似（P〉0．05），SASP组、中剂量和高剂量TGP治疗组小鼠DAI评分明显低于模型组（P均〈0．05）；模型组结肠大体评分、组织学评分、TNF—a水平和NF—kB 065的表达均显著高于正常对照组、SASP组、中剂量和高剂量TGP治疗组（P均〈0．01），与低剂量TGP治疗组相似（P〉0．05）；高剂量TGP治疗组疗效优于SASP组。结论TGP对实验性UC有一定的治疗作用，可能与其抑制NF—kB的活化，减少TNF-a的产生有关。

Effects of total glucosides of paeony on the ulcerative colitis in rats

Objective To investigate the effects of total glucosides of paeony (TGP) on mice with experimental ulcerative colitis and the underlying mechanisms. Methods Forty-eight mice were equally randomized into 6 groups ( n = 8): normal control group, model group, salicylazosulfapyridine (SASP) group, low-dose TGP group one day after enama, the mice were treated with corresponding agent by oral gavage for3 days. The disease activity index (DAI) score was evaluated every day. After all the mice were scarified, the macroscopic and histological scores were evaluated. The tumor necrosis factor-α (TNF-α) level of the colon mucosa was measured by ELISA and the colonic expression of nuclear factor-κB (NF-κB) p65 was detected by immunohistochemistry. Results Compared with the normal control group, the DAI score was significantly higher in the model group (P ＜0. 01 ),but was similar to that in low-dose TGP group ( P ＞ 0.05 ). Compared with the model group, the DAI score was significantly decreased in SASP group, medium-dose TGP group, and high-dose TGP group (all P ＜ 0. 05). The macroscopic score, histological score, TNF-α level, and expression of NF-κB p65 were significantly higher in model group than in normal control group, SASP group, medium-dose TGP group, and high-dose TGP group ( all P ＜0. 01 ), but was similar to that in low-dose TGP group (P ＞ 0. 05). The efficacy of TGP was higher in high-dose TGP group than in SASP group. Conclusions TGP has certain therapeutic effects on experimental ulcerative colitis,which may be achieved by its inhibitory effect on the activation of NF-κB and the production of TNF-α.