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| N-ras基因在K562细胞中的表达研究 | |
| 引用本文： | 燕翔,马文丽,宋艳斌,张宝,郑文岭. N-ras基因在K562细胞中的表达研究[J]. 解放军医学杂志, 2005, 30(3): 245-246 |
| 作者姓名： | 燕翔 马文丽 宋艳斌 张宝 郑文岭 |
| 作者单位： | 1. 510515,广州,南方医科大学分子生物学研究所 2. 广州军区广州总医院医学实验科 |
| 基金项目： | 国家自然科学基金资助课题(编号39880032) |
| 摘 要： | 目的探讨N-ras基因突变及表达水平与慢性粒细胞白血病发病的关系。方法以慢粒细胞株K562细胞为研究对象，采用RT-PCR和直接测序的方法对N-ras的表达水平及突变进行检测。结果RT-PCR结果示N-ras基因在K562细胞中表达升高，直接测序结果表明N-ras基因在K562细胞中不存在突变。结论N-ras基因在K562细胞中高表达，而其高表达机制与突变无关。 |
| 关 键 词： | 基因，ras K562细胞 逆转录聚合酶链反应 序列分析，DNA |
| 修稿时间： | 2004-09-23 |
A study of N-ras expression in K562 cell line |
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| Yan Xiang,Ma Wenli,Song Yanbin et al. A study of N-ras expression in K562 cell line[J]. Medical Journal of Chinese People's Liberation Army, 2005, 30(3): 245-246 | |
| Authors: | Yan Xiang Ma Wenli Song Yanbin et al |
| Affiliation: | Yan Xiang,Ma Wenli,Song Yanbin et al. Institute of Molecular Biology,Nanfang Medical University,Guangzhou 510515,China |
| Abstract: | Objective To explore the effect of the mutant and expression level of N-ras on chronic myelogenous leukemia. Method We investigated the mutant by direct sequencing in a K562 human chronic myelogenous leukemia cell line, with determination of the expression level of N-ras mRNA in K562 by RT-PCR. Result No single point mutation was detected in K562 cell line, furthermore, the expression level of N-ras gene is abnormaly high in contrast to normal human. Conclusion Our results indicated that the expression level of N-ras gene was obviously high in K562 cell line, and the underlying mechanism was not only mutation, so that further investigation is called for. |
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