| 转铁蛋白经TNP化修饰后的间接酶标检测 |
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| 引用本文： | 晏日强,周惠人,顾天爵.转铁蛋白经TNP化修饰后的间接酶标检测[J].现代免疫学,1988(6). |
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| 作者姓名： | 晏日强 周惠人 顾天爵 |
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| 作者单位： | 上海医科大学基础医学部生物化学研究室
(晏日强，周惠人)，上海医科大学基础医学部生物化学研究室(顾天爵) |
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| 摘 要： | 以三硝基苯磺酸钠(TNBS)修饰铁传递蛋白(Tf)及BSA,并制得高效价的特异性抗TNP血清。运用放射受体分析法比较Tf经TNP修饰前后与人肝癌培养细胞膜上Tf受体(TR)的亲和力,证明Tf经TNP化修饰后未改变其与受体的亲和力。应用TNP-Tf、抗TNP血清为第一抗体的间接酶标法检测人肝癌培养细胞膜上的TR,其检测敏感度是应用Tf及抗TNP血清为第一抗体的间接酶标法的4倍。以硝酸-醋酸纤维素膜作载体,用间接酶标法进行定量比较Tf及TNP-Tf的最小检测量,则Tf的最小检测限为5ng,而TNP-Tf的最小检测限为1ng。
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INDRECT IMMUNOENZYMATICAL ASSAY BY TRINITROPHENOL MODIFIED PROTEIN |
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| Abstract: | Transferrin and Bovine Albumin were modified with trmitrobenzene sulphonic acid to obtain TNP-Tf and TNP-BSA.A high titer antiserum prepared against TNP has been shown highly specific.It has been proved by analysis of radioreceptor that TNP-Tf do not affect the binding power of Tf to transferrin receptor.TNP-Tf and anti TNP antibody were used to detect TE on hepatoma cell by enzyme immunohistochemistry,and Tf/anti Tf antibody were paralleled.The sensitivity of using TNP-Tf/anti TNP wag four time higher than that of Tf/anti Tf for the detection.In immunodetection of nitrocellulose adhesive protein the minimum amount of TNP-Tf and Tf that can be detected in the dot test were 1 ng and 5 ng respectively. |
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