抑肽酶对成年豚鼠心肌再灌注损伤的保护作用

目的：探索抑肽酶的心肌保护作用。方法：建立离体心脏顺行灌注后左心做功模型，２０只成年豚鼠随机分为Ａ、Ｂ两组，分别以４℃Ｓｔ．ＴｈｏｍａｓＨｏｓｐｉｔａｌｃａｒｄｉｏｐｌｅｇｉｃｓｏｌｕｔｉｏｎＮｏ．２（ＳＴＳ）和ＳＴＳ＋抑肽酶（１５０ＫＩｕｍＬＳＴＳ）为心停搏液，测定缺血（９０分钟，２０℃）前、后和再灌注（６０分钟）时心脏动力学指标，心肌腺苷酸和丙二醛含量，并行心肌电镜观察。结果：Ｂ组再灌注后的心功能恢复，腺苷酸贮备和超微结构的改善明显高于或优于Ａ组，而丙二醛含量显著低于Ａ组（Ｐ＜０．０５）。结论：抑肽酶加入ＳＴＳ中可减少氧自由基产生，对成年豚鼠心肌缺血再灌注损伤有保护作用。

Protective effects of aprotinin on adult guinea pig myocardium subjected to ischemia reperfusion

Objective:To evaluate the protective effects of aprotinin on myocardium from ischemia reperfusion injury.Method:There were two groups(n=10)in the present study,namely,group A,in which adult guinea pig hearts were arrested by St. Thomas Hospital cardioplegic solution No.2(STS)and group B with the hearts arrested by the combination of STS and aprotinin (150KIU/ml STS).Isolated antegrade perfused left side working heart model was established .Cardiac hemodynamic parameters, contents of myocardial adenosine phosphate,malondialdehyde(MDA)were measured, myocardial ultrastructure was observed before,after ischemia and during reperfusion.Result:The recovery of cardiac function,contents of myocardial adenosine phosphate and improvement of myocardial ultrastructure after reperfusion in group B were superior to those in group A(P<0 05) Conclusion:Oxygen free radical(OFR)plays an important role in ischemia reperfusion injury to adult guinea pig myocardium.Aprotinin added to STS can effectively protect adult guinea pig myocardium from ischemia reperfusion injury by attenuating the damages of OFR.