木霉菌醇对肝癌细胞CBRH7919凋亡及形态变化的影响

目的探讨木霉菌醇(trichomerol)对大鼠肝癌细胞CBRH7919的作用。方法采用SRB细胞活性检测法检测木霉菌醇对CBRH7919细胞活性的影响;用流式细胞仪检测CBRH7919对细胞周期和凋亡的影响;激光共聚焦显微镜观察吖啶橙荧光染色细胞形态变化。结果 SRB检测显示木霉菌醇对CBRH7919细胞作用具有浓度和时间依赖性;木霉菌醇作用后CBRH7919细胞周期S期比例上调,而G1期比例下调;凋亡率具有浓度依赖性;吖啶橙荧光染色发现,细胞有明显凋亡。结论木霉菌醇对肝癌细胞CBRH7919具有良好的抑制作用,并可诱导细胞凋亡。

Effects of trichomerol on proliferation,apoptosis,mitochondrial membrane potential and morphological changes in rat clediseffect of hepatoma cell CBRH7919

Aim To explore the effect and mechanism of trichomerol on rat hepatoma cell CBRH7919.Methods The effects of rat clediseffect of hepatoma cell CBRH7919 on cell viability and cell growth were deceted by SRB method.The changes of cell cycle,cell apotosis and mitochondrial membrane potenetial were determined by flow cytometry.The morphological changes were observed under Laser scanning confocal microscope.Results The effect of rat clediseffect of hepatoma cell CBRH7919 on cell viability and cell growth was time and concentration-dependent.After treated with trichomerol,the rat clediseffect of hepatoma cell CBRH7919 in S stage was increased and in G stage was reduced.Apoptotic rate was concentration-dependent.The rat clediseffect of hepatoma cells CBRH7919 showed marked apoptosis by Acridine orange.Conclusion Trichomerol can inhibit the growth of rat hepatoma cell CBRH7919 and induce the apoptosis of cells in vitro.