Pre-S1抗原在临床及健康体检中的应用价值

目的探讨Pre-S1抗原检测的临床意义。方法采用ELISA方法检测乙肝病毒“两对半”及Pre-S1抗原,HBV-DNA检测运用荧光定量PCR方法。结果在HBsAg阳性的标本中,HBV-DNA检出率为69.3%,而Pre-S1检出率为65.8%;97例Pre-S1阳性而HBeAg阴性的标本,占Pre-S1总阳性病例的27.8%,在HBeAg阴性的病例中HBV-DNA阳性为97例,占HBV DNA总阳性病例的25.5%。在HBsAg阴性组的标本402例中,检出Pre-S1阳性9例。结论Pre-S1抗原与HBV-DNA高度相关;PreS1可替代或补充HBe系统及HBV-DNA的检测,建议纳入从业人员健康体检和献血员筛查的检测项目。

Clinical value of HBV Pre-S1 antigen detection in physical examinations

Objective To explore clinical value of HBV Pre-S1 antigen detection. Methods HBV Pre-S1 antigen and HBV markers, including HBsAg, anti-HBs, HBeAg, anti-HBe and anti-HBc, were detected by enzyme-linked immune sorbent assay (ELISA), and HBV-DNA was detected by quantitative fluorescence polymerase chain reaction (PCR).Results HBV-DNA could be detected in 69.3% of HBsAg positive specimens and HBV Pre-S1 antigen could be detected in 65.8%, with 97 of those with HBV Pre-S1 positive and HBeAg negative, accounting for 27.8% of those HBV Pre-S1 positive. HBV-DNA could be detected in 97 cases with HBeAg negative, accounting for 25.5% of those with HBV-DNA. HBV Pre-S1 was detected positive in nine of 402 cases with HBsAg positive.Conclusions HBV Pre-S1 antigen highly correlated with HBV-DNA duplication. Detection of HBe system and HBV-DNA can be supplemented and replaced by HBV Pre-S1 antigen detection. It is suggested that HBV Pre-S1 antigen should be included in the screening tests for pre-employment physical check-ups and blood donors.