黄曲霉毒素高污染区肝细胞癌p（53）蛋白与乙肝病毒感染的相关性研究

应用免疫组化ＳＰ法，检查桂西南黄曲霉毒素（ＡＦＢ１）高污染区１０９例肝细胞癌（ＨＣＣ）组织中突变型ｐ５３蛋白的表达，并与相应病例中乙型肝炎病毒表面抗原（ＨＢｓＡｇ）的表达进行相关性研究。结果发现：ＨＣＣ中ｐ５３蛋白阳性率高达６８．８％（７５／１０９），ｐ５３蛋白表达与病人性别、肿瘤大小及有无乙型肝炎病毒（ＨＢＶ）感染无关（Ｐ＞０．０５）。说明在ＡＦＢ１高污染区，ｐ５３基因突变是ＨＣＣ中非常普遍的现象。在ＨＣＣ病例中，ＨＢｓＡｇ阳性率为８４．２％（８５／１０１），其中同一病例ｐ５３蛋白及ＨＢｓＡｇ均为阳性者占５７．０％（５８／１０１），而ＨＢｓＡｇ阳性、突变型ｐ５３蛋白阴性者为２６．０％（２７／１０１）。表明ＨＢＶ仍然是肝细胞癌发生的主要原因，但ＨＢＶ的致癌机制具有多样性，并非均引起ｐ５３基因的改变。

The correlative study of mutant p53 protein and HBV infection in hepatocellular carcinomas from high aflatoxin Bl contaminated area

cases of hepatocellular carcinoma (HCC)from a high aflatoxin Bl(AFB1)contaminated area in Southwestern Guangxi were studied by immunohistochemistry,The expression of mutant p53 protein in the tumor tissues was observed and compared with the results of HBsAg examination in the same cases. p53 protein is positive in 75/109(68.8%)of the HCCs. The cases of p53 protein positive are not related to their sex, size of tumor and hepatitis B virus infection (P>0.05). This result indicates that p53 gene mutation is a very important event in HCCs of high AFB1 exposure. 85/101 (84%)cases of HCC are positive for HBsAg,in which 58 cases accompany positive expression of p53 protein. It suggests that HBV is an important risk factor for HCC, however,it is not always related to the abnormality of p53 gene.