二十岁以下慢性HBV感染者HBVDNA与HBeAg的定量关系

目的:探讨20岁以下慢性乙型肝炎病毒(HBV)感染者血清中HBV DNA、乙型肝炎病毒e抗原(HBeAg)定量之间关系.方法:用实时荧光定量聚合酶链反应(FQ-PCR)及时间分辩荧光免疫分析(TRFIA)技术检测339例(1-20岁)慢性HBV感染者血清中HBVDNA、HBeAg含量,用速率法检测ALT水平.结果:HBeAg定量＞0.3 NCU/mL、HBV DNA定量＞105 copies/mL、而ALT水平正常者占总检测病例的92.3%;HBV DNA定量(对数值)与HBeAg定量之间存在正相关关系(r=0.769,P＜0.001)和线性回归关系(b=0.32,R2=0.59,P＜0.001).结论:20岁以下慢性HBV感染者血清中HBVDNA水平与HBeAg水平存在同时消长的关系,但是有极少患者例外.HBV DNA定量与HBeAg定量两种检测方法相结合应能够更客观地反映患者HBV感染状况,二者具有互补性.

Correlation between hepatitis B virus DNA levels and HBeAg quantity in patients under 20 years old with chronic hepatitis B virus infection

AIM: To investigate the relationshipt between hepatitis B virus (HBV) DNA contents and HBeAg quantity in HBV-infected patients under 20 years old. METHODS: The serum HBV DNA contents and HBeAg quantity in 339 patients (age 1-20) with chronic HBV infection were examined by real time fluorescence quantitative polymerase chain reaction (FQ-PCR) and time-resolved fluoroim-munoassay (TRFIA) respectively, and the level of anine aminotransferase (ALT) was detected by rate method. RESULTS: The patients with HBV DNA contents more than 105 copies/ mL, HBeAg quantity more than 0.3 NCU/mL, and normal ALT level accounted for 92.3% of the total cases. Positive correlation (r = 0.769, P < 0.001) and linear regression (b = 0.32, R2 = 0.59, P < 0.001) existed between HBV DNA contents (on a log scale) and HBeAg quantity in the patients under 20 years old. CONCLUSION: Serum HBV DNA contents and HBeAg quantity are changed simultaneously in HBV-infected patients under 20 years old, except in quite a few cases, so combined examination of HBV DNA and HBeAg may help to evaluate the state of chronic HBV infection more exactly.